Assessing the inhibitory activity of oligonucleotides on TLR7 sensing

Jonathan Ferrand, Michael P. Gantier

Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Otherpeer-review

5 Citations (Scopus)

Abstract

Aberrant sensing of self-nucleic acids by Toll-like receptor (TLR) 7, 8, or 9 is associated with several autoimmune disorders, including systemic lupus erythematosus (SLE), rheumatoid arthritis, psoriasis, or systemic sclerosis. In recent years, several classes of synthetic oligonucleotides have been shown to antagonize sensing of immunostimulatory nucleic acids by TLR7/8/9, indicating that these molecules could have therapeutic applications in such autoimmune diseases. Conversely, synthetic oligonucleotides used in therapeutic technologies such as antisense and microRNA inhibitors also have the potential to inhibit TLR7/8/9 sensing, rendering patients more susceptible to viral/bacterial infections. This chapter describes a protocol to define the inhibitory activity of synthetic oligonucleotides on TLR7.

Original languageEnglish
Title of host publicationToll-Like Receptors
Subtitle of host publicationPractice and Methods
EditorsClaire E McCoy
Place of PublicationNew York NY USA
PublisherHumana Press
Pages79-90
Number of pages12
Edition2nd
ISBN (Electronic)978-1-4939-3335-8
ISBN (Print)978-1-4939-3333-4
DOIs
Publication statusPublished - 1 Jan 2016

Publication series

NameMethods in Molecular Biology: Springer Protocols
PublisherSpringer
Number1390
ISSN (Print)10643745
ISSN (Electronic)1940-6029

Keywords

  • Innate immunity
  • Oligonucleotides
  • TLR7
  • TLR8
  • Toll-like receptors

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