Assessing the circulating placental-specific anti-angiogenic protein sFLT-1 e15a in preeclampsia

Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Other

3 Citations (Scopus)

Abstract

Preeclampsia is a common obstetric complication globally responsible for a significant burden of maternal and perinatal morbidity and mortality. The anti-angiogenic protein, sFLT-1, plays a central role in its pathophysiology. sFLT-1 is released from a range of tissues into the circulation, where it antagonizes the activity of vascular endothelial growth factor and placental growth factor leading to endothelial dysfunction. The resulting widespread endothelial dysfunction produces the clinical features of preeclampsia including hypertension and proteinuria. Multiple splice variants of sFLT-1 have been identified, with one, known as sFLT-1 e15a, present only in humans and higher-order primates. This sFLT-1 variant is also the main form of sFLT-1 produced by the placenta. Recent work has shown that sFLT-1 e15a is significantly elevated in the placenta and circulation of women with preeclampsia. It is also biologically active, capable of causing endothelial dysfunction and end-organ dysfunction seen in preeclampsia. Indeed, overexpression of sFLT-1 e15a in mice recapitulates the preeclamptic phenotype in pregnancy. No commercial assay currently exists to analyze sFLT-1 e15a protein levels. Here, a new ELISA method to determine circulating sFLT-1 variant levels is described.

Original languageEnglish
Title of host publicationPreeclampsia: Methods and Protocols
Subtitle of host publicationMethods in Molecular Biology
EditorsPadma Murthi, Cathy Vaillancourt
Place of PublicationNY USA
PublisherHumana Press
Chapter3
Pages27-37
Number of pages11
ISBN (Print)978-1-4939-7497-9
DOIs
Publication statusPublished - 1 Jan 2018

Publication series

NameMethods in Molecular Biology
Volume1710
ISSN (Print)1064-3745

Keywords

  • ELISA
  • Immunoassay
  • Preeclampsia
  • Protein
  • sFLT-1
  • Splice variant

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