Assessing the cGAS-cGAMP-STING activity of cancer cells

Geneviève Pépin, Michael P. Gantier

Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Otherpeer-review

2 Citations (Scopus)

Abstract

DNA sensing by the STING pathway is emerging to be a crucial component of the antitumor immune response. Although it plays a key role in the activation of tumor immune cells, exactly how STING is activated by tumor cells is not fully understood. Recent evidence suggests that cGAS can be directly engaged and produces 2′3′-cyclic-GMP-AMP (cGAMP) within certain tumor cells upon stimulation with DNA damaging agents. Because cGAMP can transfer between adjacent cells, the capacity of tumor cells to produce cGAMP may activate tumor immune cells, even in the absence of functional STING signaling within the tumor. Here we describe a simple coculture protocol allowing for the functional characterization of cGAS/STING activity in tumor cells, together with cGAMP transfer to adjacent cells. This approach will help define how different tumors engage the STING pathway, and whether synthetic STING agonists should be used to potentiate the antitumor effects of chemotherapies.

Original languageEnglish
Title of host publicationInflammation and Cancer
Subtitle of host publicationMethods and Protocols
EditorsBrendan J. Jenkins
Place of PublicationNew York, NY
PublisherHumana Press
Chapter20
Pages257-266
Number of pages10
ISBN (Electronic)9781493975686
ISBN (Print)9781493975679
DOIs
Publication statusPublished - 1 Jan 2018

Publication series

NameMethods in Molecular Biology
Volume1725
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Cancer
  • cGAMP
  • cGAS
  • Connexin
  • Immunotherapy
  • Innate immunity
  • Interferon
  • STING

Cite this

Pépin, G., & Gantier, M. P. (2018). Assessing the cGAS-cGAMP-STING activity of cancer cells. In B. J. Jenkins (Ed.), Inflammation and Cancer: Methods and Protocols (pp. 257-266). (Methods in Molecular Biology; Vol. 1725). Humana Press. https://doi.org/10.1007/978-1-4939-7568-6_20