Assessing gut microbiome-liver crosstalk with a modular microfluidic platform

Hsih Yin Tan; Louis Jun Ye Ong; Chak Ming Leung; Lor Huai Chong; Yi Chin Toh

Assessing gut microbiome-liver crosstalk with a modular microfluidic platform

Hsih Yin Tan, Louis Jun Ye Ong, Chak Ming Leung, Lor Huai Chong, Yi Chin Toh

Research output: Chapter in Book/Report/Conference proceeding › Conference Paper › Other

Abstract

We report a compartmentalized microbiome module that allows culturing of commensal bacteria under either aerobic or anaerobic conditions, that can be easily connected to our existing modular microfluidic platform. The microbiome module is designed to permit translocation of metabolites across a semi-permeable membrane and circulated to a 3D liver culture module. Our findings showed that microbial metabolites such as sodium butyrate (NaB) can reduce lipid droplet accumulation and pro-inflammation markers in fatty liver cultures.

Original language	English
Title of host publication	23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
Publisher	Chemical and Biological Microsystems Society
Pages	56-57
Number of pages	2
ISBN (Electronic)	9781733419000
Publication status	Published - 2019
Externally published	Yes
Event	International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2019) - Congress Center Basel, Basel, Switzerland Duration: 27 Oct 2019 → 31 Oct 2019 Conference number: 23rd https://microtas2019.org/

Conference

Conference	International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2019)
Abbreviated title	MicroTAS 2019
Country/Territory	Switzerland
City	Basel
Period	27/10/19 → 31/10/19
Other	The 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences (µTAS 2019) 27 - 31 October 2019 Conference Chairs: Petra Dittrich - ETH Zürich, SWITZERLAND Andreas Hierlemann - ETH Zürich, SWITZERLAND Emmanuel Delamarche - IBM Research - Zürich, SWITZERLAND Conference Location: Congress Center Basel Basel, SWITZERLAND www.congress.ch
Internet address	https://microtas2019.org/

Keywords

Fatty liver
Gut metabolites
Microbiome-liver crosstalk
Modular microfluidics
Organs-on-chips

Cite this

@inproceedings{edcd34e042e645708e2faa80a805d006,

title = "Assessing gut microbiome-liver crosstalk with a modular microfluidic platform",

abstract = "We report a compartmentalized microbiome module that allows culturing of commensal bacteria under either aerobic or anaerobic conditions, that can be easily connected to our existing modular microfluidic platform. The microbiome module is designed to permit translocation of metabolites across a semi-permeable membrane and circulated to a 3D liver culture module. Our findings showed that microbial metabolites such as sodium butyrate (NaB) can reduce lipid droplet accumulation and pro-inflammation markers in fatty liver cultures.",

keywords = "Fatty liver, Gut metabolites, Microbiome-liver crosstalk, Modular microfluidics, Organs-on-chips",

author = "Tan, {Hsih Yin} and Ong, {Louis Jun Ye} and Leung, {Chak Ming} and Chong, {Lor Huai} and Toh, {Yi Chin}",

note = "Funding Information: This work is supported by SMART Innovation Grant (R-722-000-013-592), Institute for Health Innovation and Technology, NI Institute and NUS Tissue Engineering Programme. Publisher Copyright: {\textcopyright} 2019 CBMS-0001.; International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2019), MicroTAS 2019 ; Conference date: 27-10-2019 Through 31-10-2019",

year = "2019",

language = "English",

pages = "56--57",

booktitle = "23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019",

publisher = "Chemical and Biological Microsystems Society",

address = "United States of America",

url = "https://microtas2019.org/",

}

Tan, HY, Ong, LJY, Leung, CM, Chong, LH & Toh, YC 2019, Assessing gut microbiome-liver crosstalk with a modular microfluidic platform. in 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019. Chemical and Biological Microsystems Society, pp. 56-57, International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2019), Basel, Switzerland, 27/10/19.

Assessing gut microbiome-liver crosstalk with a modular microfluidic platform. / Tan, Hsih Yin; Ong, Louis Jun Ye; Leung, Chak Ming et al.
23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019. Chemical and Biological Microsystems Society, 2019. p. 56-57.

Research output: Chapter in Book/Report/Conference proceeding › Conference Paper › Other

TY - GEN

T1 - Assessing gut microbiome-liver crosstalk with a modular microfluidic platform

AU - Tan, Hsih Yin

AU - Ong, Louis Jun Ye

AU - Leung, Chak Ming

AU - Chong, Lor Huai

AU - Toh, Yi Chin

N1 - Conference code: 23rd

PY - 2019

Y1 - 2019

N2 - We report a compartmentalized microbiome module that allows culturing of commensal bacteria under either aerobic or anaerobic conditions, that can be easily connected to our existing modular microfluidic platform. The microbiome module is designed to permit translocation of metabolites across a semi-permeable membrane and circulated to a 3D liver culture module. Our findings showed that microbial metabolites such as sodium butyrate (NaB) can reduce lipid droplet accumulation and pro-inflammation markers in fatty liver cultures.

AB - We report a compartmentalized microbiome module that allows culturing of commensal bacteria under either aerobic or anaerobic conditions, that can be easily connected to our existing modular microfluidic platform. The microbiome module is designed to permit translocation of metabolites across a semi-permeable membrane and circulated to a 3D liver culture module. Our findings showed that microbial metabolites such as sodium butyrate (NaB) can reduce lipid droplet accumulation and pro-inflammation markers in fatty liver cultures.

KW - Fatty liver

KW - Gut metabolites

KW - Microbiome-liver crosstalk

KW - Modular microfluidics

KW - Organs-on-chips

UR - http://www.scopus.com/inward/record.url?scp=85094967208&partnerID=8YFLogxK

M3 - Conference Paper

AN - SCOPUS:85094967208

SP - 56

EP - 57

BT - 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019

PB - Chemical and Biological Microsystems Society

T2 - International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2019)

Y2 - 27 October 2019 through 31 October 2019

ER -

Assessing gut microbiome-liver crosstalk with a modular microfluidic platform

Abstract

Conference

Keywords

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Cite this