Abstract
| Original language | English |
|---|---|
| Title of host publication | Methods in Molecular Biology (Methods and Protocols) |
| Publisher | Springer |
| Pages | 207-217 |
| Volume | 687 |
| Publication status | Published - 2011 |
Publication series
| Name | PCR Protocols |
|---|
Cite this
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Assessing gene-specific methylation using HRM-based analysis. / Wong, Ee Ming; Dobrovic, Alexander N.
Methods in Molecular Biology (Methods and Protocols). Vol. 687 Springer, 2011. p. 207-217 (PCR Protocols).Research output: Chapter in Book/Report/Conference proceeding › Chapter (Book) › Other
TY - CHAP
T1 - Assessing gene-specific methylation using HRM-based analysis.
AU - Wong, Ee Ming
AU - Dobrovic, Alexander N
PY - 2011
Y1 - 2011
N2 - As DNA methylation analysis enters the mainstream of biomedical research, there is increasing interest in methodologies that can be used for rapid analysis of a wide variety of biological and clinical samples. The methods that have been commonly used for methylation analysis are usually time-consuming and require multiple steps. The methylation-sensitive high-resolution melting (MS-HRM) assay is an in-tube assay which was developed to assess promoter methylation in sodium bisulphite-modified DNA and is particularly useful for assessing methylation in short fragments of DNA derived from formalin-fixed paraffin-embedded biopsies.
AB - As DNA methylation analysis enters the mainstream of biomedical research, there is increasing interest in methodologies that can be used for rapid analysis of a wide variety of biological and clinical samples. The methods that have been commonly used for methylation analysis are usually time-consuming and require multiple steps. The methylation-sensitive high-resolution melting (MS-HRM) assay is an in-tube assay which was developed to assess promoter methylation in sodium bisulphite-modified DNA and is particularly useful for assessing methylation in short fragments of DNA derived from formalin-fixed paraffin-embedded biopsies.
M3 - Chapter (Book)
VL - 687
T3 - PCR Protocols
SP - 207
EP - 217
BT - Methods in Molecular Biology (Methods and Protocols)
PB - Springer
ER -