Assessing gene-specific methylation using HRM-based analysis.

Ee Ming Wong, Alexander N Dobrovic

Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Other

Abstract

As DNA methylation analysis enters the mainstream of biomedical research, there is increasing interest in methodologies that can be used for rapid analysis of a wide variety of biological and clinical samples. The methods that have been commonly used for methylation analysis are usually time-consuming and require multiple steps. The methylation-sensitive high-resolution melting (MS-HRM) assay is an in-tube assay which was developed to assess promoter methylation in sodium bisulphite-modified DNA and is particularly useful for assessing methylation in short fragments of DNA derived from formalin-fixed paraffin-embedded biopsies.
Original languageEnglish
Title of host publicationMethods in Molecular Biology (Methods and Protocols)
PublisherSpringer
Pages207-217
Volume687
Publication statusPublished - 2011

Publication series

NamePCR Protocols

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