Assessing gene-specific methylation using HRM-based analysis.

Ee Ming Wong, Alexander N Dobrovic

Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Other

Abstract

As DNA methylation analysis enters the mainstream of biomedical research, there is increasing interest in methodologies that can be used for rapid analysis of a wide variety of biological and clinical samples. The methods that have been commonly used for methylation analysis are usually time-consuming and require multiple steps. The methylation-sensitive high-resolution melting (MS-HRM) assay is an in-tube assay which was developed to assess promoter methylation in sodium bisulphite-modified DNA and is particularly useful for assessing methylation in short fragments of DNA derived from formalin-fixed paraffin-embedded biopsies.
Original languageEnglish
Title of host publicationMethods in Molecular Biology (Methods and Protocols)
PublisherSpringer
Pages207-217
Volume687
Publication statusPublished - 2011

Publication series

NamePCR Protocols

Cite this

Wong, E. M., & Dobrovic, A. N. (2011). Assessing gene-specific methylation using HRM-based analysis. In Methods in Molecular Biology (Methods and Protocols) (Vol. 687, pp. 207-217). (PCR Protocols). Springer.
Wong, Ee Ming ; Dobrovic, Alexander N. / Assessing gene-specific methylation using HRM-based analysis. Methods in Molecular Biology (Methods and Protocols). Vol. 687 Springer, 2011. pp. 207-217 (PCR Protocols).
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Wong, EM & Dobrovic, AN 2011, Assessing gene-specific methylation using HRM-based analysis. in Methods in Molecular Biology (Methods and Protocols). vol. 687, PCR Protocols, Springer, pp. 207-217.

Assessing gene-specific methylation using HRM-based analysis. / Wong, Ee Ming; Dobrovic, Alexander N.

Methods in Molecular Biology (Methods and Protocols). Vol. 687 Springer, 2011. p. 207-217 (PCR Protocols).

Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Other

TY - CHAP

T1 - Assessing gene-specific methylation using HRM-based analysis.

AU - Wong, Ee Ming

AU - Dobrovic, Alexander N

PY - 2011

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AB - As DNA methylation analysis enters the mainstream of biomedical research, there is increasing interest in methodologies that can be used for rapid analysis of a wide variety of biological and clinical samples. The methods that have been commonly used for methylation analysis are usually time-consuming and require multiple steps. The methylation-sensitive high-resolution melting (MS-HRM) assay is an in-tube assay which was developed to assess promoter methylation in sodium bisulphite-modified DNA and is particularly useful for assessing methylation in short fragments of DNA derived from formalin-fixed paraffin-embedded biopsies.

M3 - Chapter (Book)

VL - 687

T3 - PCR Protocols

SP - 207

EP - 217

BT - Methods in Molecular Biology (Methods and Protocols)

PB - Springer

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Wong EM, Dobrovic AN. Assessing gene-specific methylation using HRM-based analysis. In Methods in Molecular Biology (Methods and Protocols). Vol. 687. Springer. 2011. p. 207-217. (PCR Protocols).