Assembly of poly(dopamine)/poly(N -isopropylacrylamide) mixed films and their temperature-dependent interaction with proteins, liposomes, and cells

Yan Zhang, Karthiga Panneerselvam, Ryosuke Ogaki, Leticia Hosta-Rigau, Rebecca Van Der Westen, Bettina E B Jensen, Boon M. Teo, Meifang Zhu, Brigitte Städler

Research output: Contribution to journalArticleResearchpeer-review

30 Citations (Scopus)


Many biomedical applications benefit from responsive polymer coatings. The properties of poly(dopamine) (PDA) films can be affected by codepositing dopamine (DA) with the temperature-responsive polymer poly(N- isopropylacrylamide) (pNiPAAm). We characterize the film assembly at 24 and 39 C using DA and aminated or carboxylated pNiPAAm by a quartz crystal microbalance with dissipation monitoring (QCM-D), X-ray photoelectron spectroscopy, UV-vis, ellipsometry, and atomic force microscopy. It was found that pNiPAAm with both types of end groups are incorporated into the films. We then identified a temperature-dependent adsorption behavior of proteins and liposomes to these PDA and pNiPAAm containing coatings by QCM-D and optical microscopy. Finally, a difference in myoblast cell response was found when these cells were allowed to adhere to these coatings. Taken together, these fundamental findings considerably broaden the potential biomedical applications of PDA films due to the added temperature responsiveness.

Original languageEnglish
Pages (from-to)10213-10222
Number of pages10
Issue number32
Publication statusPublished - 13 Aug 2013
Externally publishedYes

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