TY - JOUR
T1 - Assembly of poly(dopamine)/poly(N -isopropylacrylamide) mixed films and their temperature-dependent interaction with proteins, liposomes, and cells
AU - Zhang, Yan
AU - Panneerselvam, Karthiga
AU - Ogaki, Ryosuke
AU - Hosta-Rigau, Leticia
AU - Van Der Westen, Rebecca
AU - Jensen, Bettina E B
AU - Teo, Boon M.
AU - Zhu, Meifang
AU - Städler, Brigitte
PY - 2013/8/13
Y1 - 2013/8/13
N2 - Many biomedical applications benefit from responsive polymer coatings. The properties of poly(dopamine) (PDA) films can be affected by codepositing dopamine (DA) with the temperature-responsive polymer poly(N- isopropylacrylamide) (pNiPAAm). We characterize the film assembly at 24 and 39 C using DA and aminated or carboxylated pNiPAAm by a quartz crystal microbalance with dissipation monitoring (QCM-D), X-ray photoelectron spectroscopy, UV-vis, ellipsometry, and atomic force microscopy. It was found that pNiPAAm with both types of end groups are incorporated into the films. We then identified a temperature-dependent adsorption behavior of proteins and liposomes to these PDA and pNiPAAm containing coatings by QCM-D and optical microscopy. Finally, a difference in myoblast cell response was found when these cells were allowed to adhere to these coatings. Taken together, these fundamental findings considerably broaden the potential biomedical applications of PDA films due to the added temperature responsiveness.
AB - Many biomedical applications benefit from responsive polymer coatings. The properties of poly(dopamine) (PDA) films can be affected by codepositing dopamine (DA) with the temperature-responsive polymer poly(N- isopropylacrylamide) (pNiPAAm). We characterize the film assembly at 24 and 39 C using DA and aminated or carboxylated pNiPAAm by a quartz crystal microbalance with dissipation monitoring (QCM-D), X-ray photoelectron spectroscopy, UV-vis, ellipsometry, and atomic force microscopy. It was found that pNiPAAm with both types of end groups are incorporated into the films. We then identified a temperature-dependent adsorption behavior of proteins and liposomes to these PDA and pNiPAAm containing coatings by QCM-D and optical microscopy. Finally, a difference in myoblast cell response was found when these cells were allowed to adhere to these coatings. Taken together, these fundamental findings considerably broaden the potential biomedical applications of PDA films due to the added temperature responsiveness.
UR - http://www.scopus.com/inward/record.url?scp=84881566195&partnerID=8YFLogxK
U2 - 10.1021/la402118u
DO - 10.1021/la402118u
M3 - Article
AN - SCOPUS:84881566195
VL - 29
SP - 10213
EP - 10222
JO - Langmuir
JF - Langmuir
SN - 0743-7463
IS - 32
ER -