Assembly of beta-barrel proteins into bacterial outer membranes

Joel Pearson Selkrig, Denisse Lorena Leyton, Chaille Webb, Trevor James Lithgow

Research output: Contribution to journalArticleResearchpeer-review

52 Citations (Scopus)

Abstract

Membrane proteins with a beta-barrel topology are found in the outer membranes of Gram-negative bacteria and in the plastids and mitochondria of eukaryotic cells. The assembly of these membrane proteins depends on a protein folding reaction (to create the barrel) and an insertion reaction (to integrate the barrel within the outer membrane). Experimental approaches using biophysics and biochemistry are detailing the steps in the assembly pathway, while genetics and bioinformatics have revealed a sophisticated production line of cellular components that catalyze the assembly pathway in vivo. This includes the modular BAM complex, several molecular chaperones and the translocation and assembly module (the TAM). Recent screens also suggest that further components of the pathway might remain to be discovered. We review what is known about the process of beta-barrel protein assembly into membranes, and the components of the beta-barrel assembly machinery. This article is part of a Special Issue entitled: Protein trafficking and secretion in bacteria. Guest Editors: Anastassios Economou and Ross Dalbey.
Original languageEnglish
Pages (from-to)1542 - 1550
Number of pages9
JournalBiochimica et Biophysica Acta - Molecular Cell Research
Volume1843
Issue number8
DOIs
Publication statusPublished - 2014

Cite this

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title = "Assembly of beta-barrel proteins into bacterial outer membranes",
abstract = "Membrane proteins with a beta-barrel topology are found in the outer membranes of Gram-negative bacteria and in the plastids and mitochondria of eukaryotic cells. The assembly of these membrane proteins depends on a protein folding reaction (to create the barrel) and an insertion reaction (to integrate the barrel within the outer membrane). Experimental approaches using biophysics and biochemistry are detailing the steps in the assembly pathway, while genetics and bioinformatics have revealed a sophisticated production line of cellular components that catalyze the assembly pathway in vivo. This includes the modular BAM complex, several molecular chaperones and the translocation and assembly module (the TAM). Recent screens also suggest that further components of the pathway might remain to be discovered. We review what is known about the process of beta-barrel protein assembly into membranes, and the components of the beta-barrel assembly machinery. This article is part of a Special Issue entitled: Protein trafficking and secretion in bacteria. Guest Editors: Anastassios Economou and Ross Dalbey.",
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Assembly of beta-barrel proteins into bacterial outer membranes. / Selkrig, Joel Pearson; Leyton, Denisse Lorena; Webb, Chaille; Lithgow, Trevor James.

In: Biochimica et Biophysica Acta - Molecular Cell Research, Vol. 1843, No. 8, 2014, p. 1542 - 1550.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Assembly of beta-barrel proteins into bacterial outer membranes

AU - Selkrig, Joel Pearson

AU - Leyton, Denisse Lorena

AU - Webb, Chaille

AU - Lithgow, Trevor James

PY - 2014

Y1 - 2014

N2 - Membrane proteins with a beta-barrel topology are found in the outer membranes of Gram-negative bacteria and in the plastids and mitochondria of eukaryotic cells. The assembly of these membrane proteins depends on a protein folding reaction (to create the barrel) and an insertion reaction (to integrate the barrel within the outer membrane). Experimental approaches using biophysics and biochemistry are detailing the steps in the assembly pathway, while genetics and bioinformatics have revealed a sophisticated production line of cellular components that catalyze the assembly pathway in vivo. This includes the modular BAM complex, several molecular chaperones and the translocation and assembly module (the TAM). Recent screens also suggest that further components of the pathway might remain to be discovered. We review what is known about the process of beta-barrel protein assembly into membranes, and the components of the beta-barrel assembly machinery. This article is part of a Special Issue entitled: Protein trafficking and secretion in bacteria. Guest Editors: Anastassios Economou and Ross Dalbey.

AB - Membrane proteins with a beta-barrel topology are found in the outer membranes of Gram-negative bacteria and in the plastids and mitochondria of eukaryotic cells. The assembly of these membrane proteins depends on a protein folding reaction (to create the barrel) and an insertion reaction (to integrate the barrel within the outer membrane). Experimental approaches using biophysics and biochemistry are detailing the steps in the assembly pathway, while genetics and bioinformatics have revealed a sophisticated production line of cellular components that catalyze the assembly pathway in vivo. This includes the modular BAM complex, several molecular chaperones and the translocation and assembly module (the TAM). Recent screens also suggest that further components of the pathway might remain to be discovered. We review what is known about the process of beta-barrel protein assembly into membranes, and the components of the beta-barrel assembly machinery. This article is part of a Special Issue entitled: Protein trafficking and secretion in bacteria. Guest Editors: Anastassios Economou and Ross Dalbey.

UR - http://www.ncbi.nlm.nih.gov/pubmed/24135059

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JO - Biochimica et Biophysica Acta - Molecular Cell Research

JF - Biochimica et Biophysica Acta - Molecular Cell Research

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