Assays for Inducing and Measuring Cell Death to Detect Macrophage Migration Inhibitory Factor (MIF) Release

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Abstract

Cell death is a vital process for maintaining tissue homeostasis and removing potentially harmful cells. Cell death can be both programmed and non-programmed and is commonly divided into two main forms, termed apoptotic and necrotic death modes. In this chapter cell death is classified into apoptosis, primary necrosis, pyroptosis, and necroptosis. This chapter outlines the measurement of these different types of cell death and the relationship of measuring MIF release in these assays.

Original languageEnglish
Pages (from-to)173-183
Number of pages11
JournalMethods in molecular biology (Clifton, N.J.)
Volume2080
DOIs
Publication statusPublished - 1 Jan 2020

Keywords

  • Apoptosis
  • Colorimetric assay
  • Flow cytometry
  • MIF
  • Necroptosis
  • Necrosis
  • Pyroptosis

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