TY - JOUR
T1 - Asgard archaea modulate potential methanogenesis substrates in wetland soil
AU - Valentin-Alvarado, Luis E.
AU - Appler, Kathryn E.
AU - De Anda, Valerie
AU - Schoelmerich, Marie C.
AU - West-Roberts, Jacob
AU - Kivenson, Veronika
AU - Crits-Christoph, Alexander
AU - Ly, Lynn
AU - Sachdeva, Rohan
AU - Greening, Chris
AU - Savage, David F.
AU - Baker, Brett J.
AU - Banfield, Jillian F.
N1 - Publisher Copyright:
© The Author(s) 2024.
PY - 2024/7/31
Y1 - 2024/7/31
N2 - The roles of Asgard archaea in eukaryogenesis and marine biogeochemical cycles are well studied, yet their contributions in soil ecosystems remain unknown. Of particular interest are Asgard archaeal contributions to methane cycling in wetland soils. To investigate this, we reconstructed two complete genomes for soil-associated Atabeyarchaeia, a new Asgard lineage, and a complete genome of Freyarchaeia, and predicted their metabolism in situ. Metatranscriptomics reveals expression of genes for [NiFe]-hydrogenases, pyruvate oxidation and carbon fixation via the Wood-Ljungdahl pathway. Also expressed are genes encoding enzymes for amino acid metabolism, anaerobic aldehyde oxidation, hydrogen peroxide detoxification and carbohydrate breakdown to acetate and formate. Overall, soil-associated Asgard archaea are predicted to include non-methanogenic acetogens, highlighting their potential role in carbon cycling in terrestrial environments.
AB - The roles of Asgard archaea in eukaryogenesis and marine biogeochemical cycles are well studied, yet their contributions in soil ecosystems remain unknown. Of particular interest are Asgard archaeal contributions to methane cycling in wetland soils. To investigate this, we reconstructed two complete genomes for soil-associated Atabeyarchaeia, a new Asgard lineage, and a complete genome of Freyarchaeia, and predicted their metabolism in situ. Metatranscriptomics reveals expression of genes for [NiFe]-hydrogenases, pyruvate oxidation and carbon fixation via the Wood-Ljungdahl pathway. Also expressed are genes encoding enzymes for amino acid metabolism, anaerobic aldehyde oxidation, hydrogen peroxide detoxification and carbohydrate breakdown to acetate and formate. Overall, soil-associated Asgard archaea are predicted to include non-methanogenic acetogens, highlighting their potential role in carbon cycling in terrestrial environments.
UR - http://www.scopus.com/inward/record.url?scp=85200052136&partnerID=8YFLogxK
U2 - 10.1038/s41467-024-49872-z
DO - 10.1038/s41467-024-49872-z
M3 - Article
C2 - 39085194
AN - SCOPUS:85200052136
SN - 2041-1723
VL - 15
JO - Nature Communications
JF - Nature Communications
IS - 1
M1 - 6384
ER -
