TY - JOUR
T1 - Aromatase and prostaglandin inter-relationships in breast adipose tissue
T2 - Significance for breast cancer development
AU - Karuppu, Darshini
AU - Kalus, Alan
AU - Simpson, Evan R.
AU - Clyne, Colin
PY - 2002/11
Y1 - 2002/11
N2 - The role of prostaglandins in the development of breast cancer is a topic of growing interest. Stimulation of aromatase expression within the breast has been proposed as a mechanism whereby prostaglandins could influence breast cancer growth. In the present study, we show that PGE2 is a powerful stimulator of aromatase expression in human breast adipose stromal cells. Moreover, TNFα, which also stimulates aromatase expression in breast adipose stromal cells, acts to increase the secretion of PGE2 by these cells, as well as the expression of COX 2 and PGE synthase, but not that of COX 1. On the other hand, class I cytokines had no effect, either by themselves or in the presence of estradiol. These factors had little influence on secretion of 15-deoxy-Δ12.14-PGJ2, which is inhibitory of aromatase expression by breast adipose stromal cells. These results further substantiate an important role for PGE2 to stimulate estrogen biosynthesis within the local environment of the breast.
AB - The role of prostaglandins in the development of breast cancer is a topic of growing interest. Stimulation of aromatase expression within the breast has been proposed as a mechanism whereby prostaglandins could influence breast cancer growth. In the present study, we show that PGE2 is a powerful stimulator of aromatase expression in human breast adipose stromal cells. Moreover, TNFα, which also stimulates aromatase expression in breast adipose stromal cells, acts to increase the secretion of PGE2 by these cells, as well as the expression of COX 2 and PGE synthase, but not that of COX 1. On the other hand, class I cytokines had no effect, either by themselves or in the presence of estradiol. These factors had little influence on secretion of 15-deoxy-Δ12.14-PGJ2, which is inhibitory of aromatase expression by breast adipose stromal cells. These results further substantiate an important role for PGE2 to stimulate estrogen biosynthesis within the local environment of the breast.
KW - Aromatase
KW - Breast cancer
KW - Breast mesenchymal cells
KW - Prostaglandin E
KW - TNFα
UR - http://www.scopus.com/inward/record.url?scp=0036829273&partnerID=8YFLogxK
U2 - 10.1023/A:1020531329686
DO - 10.1023/A:1020531329686
M3 - Article
C2 - 12452446
AN - SCOPUS:0036829273
SN - 0167-6806
VL - 76
SP - 103
EP - 109
JO - Breast Cancer Research and Treatment
JF - Breast Cancer Research and Treatment
IS - 2
ER -