Arid3b is essential for second heart field cell deployment and heart patterning

Veronica Uribe, Claudio Badia Careaga, Jesus C Fernandez Casanova, Jorge Nicolas Dominguez Macias, Jose Luis de la Pompa, Juan Jose Sanz-Ezquerro

Research output: Contribution to journalArticleResearchpeer-review

7 Citations (Scopus)

Abstract

Arid3b, a member of the conserved ARID family of transcription factors, is essential for mouse embryonic development but its precise roles are poorly understood. Here, we show that Arid3b is expressed in the myocardium of the tubular heart and in second heart field progenitors. Arid3b-deficient embryos show cardiac abnormalities, including a notable shortening of the poles, absence of myocardial differentiation and altered patterning of the atrioventricular canal, which also lacks epithelial-to-mesenchymal transition. Proliferation and death of progenitors as well as early patterning of the heart appear normal. However, DiI labelling of second heart field progenitors revealed a defect in the addition of cells to the heart. RNA microarray analysis uncovered a set of differentially expressed genes in Arid3b-deficient tissues, including Bhlhb2, a regulator of cardiomyocyte differentiation, and Lims2, a gene involved in cell migration. Arid3b is thus required for heart development by regulating the motility and differentiation of heart progenitors. These findings identify Arid3b as a candidate gene involved in the aetiology of human congenital malformations.
Original languageEnglish
Pages (from-to)4168 - 4181
Number of pages14
JournalDevelopment
Volume141
Issue number21
DOIs
Publication statusPublished - 2014

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