Aptamer-DNAzyme hairpins for biosensing of Ochratoxin A

Cheng Yang; Vasilica Lates; Beatriz Prieto-Simón; Jean Louis Marty; Xiurong Yang

doi:10.1016/j.bios.2011.12.011

Aptamer-DNAzyme hairpins for biosensing of Ochratoxin A

Cheng Yang
, Vasilica Lates
, Beatriz Prieto-Simón
, Jean Louis Marty
, Xiurong Yang

Research output: Contribution to journal › Article › Research › peer-review

140 Citations (Scopus)

Abstract

We report an aptasensor for biosensing of Ochratoxin A (OTA) using aptamer-DNAzyme hairpin as biorecognition element. The structure of this engineered nucleic acid includes the horseradish peroxidase (HRP)-mimicking DNAzyme and the OTA specific aptamer sequences. A blocking tail captures a part of these sequences in the stem region of the hairpin. In the presence of OTA, the hairpin is opened due to the formation of the aptamer-analyte complex. As a result, self-assembly of the active HRP-mimicking DNAzyme occurs. The activity of this DNAzyme is linearly correlated with OTA concentration up to 10. nM, showing a limit of detection of 2.5. nM.

Original language	English
Pages (from-to)	208-212
Number of pages	5
Journal	Biosensors and Bioelectronics
Volume	32
Issue number	1
DOIs	https://doi.org/10.1016/j.bios.2011.12.011
Publication status	Published - 15 Feb 2012
Externally published	Yes

Keywords

Aptamer
DNAzyme
G-quadruplex
Hairpin
Ochratoxin A

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10.1016/j.bios.2011.12.011

Cite this

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title = "Aptamer-DNAzyme hairpins for biosensing of Ochratoxin A",

abstract = "We report an aptasensor for biosensing of Ochratoxin A (OTA) using aptamer-DNAzyme hairpin as biorecognition element. The structure of this engineered nucleic acid includes the horseradish peroxidase (HRP)-mimicking DNAzyme and the OTA specific aptamer sequences. A blocking tail captures a part of these sequences in the stem region of the hairpin. In the presence of OTA, the hairpin is opened due to the formation of the aptamer-analyte complex. As a result, self-assembly of the active HRP-mimicking DNAzyme occurs. The activity of this DNAzyme is linearly correlated with OTA concentration up to 10. nM, showing a limit of detection of 2.5. nM.",

keywords = "Aptamer, DNAzyme, G-quadruplex, Hairpin, Ochratoxin A",

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doi = "10.1016/j.bios.2011.12.011",

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T1 - Aptamer-DNAzyme hairpins for biosensing of Ochratoxin A

AU - Yang, Cheng

AU - Lates, Vasilica

AU - Prieto-Simón, Beatriz

AU - Marty, Jean Louis

AU - Yang, Xiurong

PY - 2012/2/15

Y1 - 2012/2/15

N2 - We report an aptasensor for biosensing of Ochratoxin A (OTA) using aptamer-DNAzyme hairpin as biorecognition element. The structure of this engineered nucleic acid includes the horseradish peroxidase (HRP)-mimicking DNAzyme and the OTA specific aptamer sequences. A blocking tail captures a part of these sequences in the stem region of the hairpin. In the presence of OTA, the hairpin is opened due to the formation of the aptamer-analyte complex. As a result, self-assembly of the active HRP-mimicking DNAzyme occurs. The activity of this DNAzyme is linearly correlated with OTA concentration up to 10. nM, showing a limit of detection of 2.5. nM.

AB - We report an aptasensor for biosensing of Ochratoxin A (OTA) using aptamer-DNAzyme hairpin as biorecognition element. The structure of this engineered nucleic acid includes the horseradish peroxidase (HRP)-mimicking DNAzyme and the OTA specific aptamer sequences. A blocking tail captures a part of these sequences in the stem region of the hairpin. In the presence of OTA, the hairpin is opened due to the formation of the aptamer-analyte complex. As a result, self-assembly of the active HRP-mimicking DNAzyme occurs. The activity of this DNAzyme is linearly correlated with OTA concentration up to 10. nM, showing a limit of detection of 2.5. nM.

KW - Aptamer

KW - DNAzyme

KW - G-quadruplex

KW - Hairpin

KW - Ochratoxin A

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DO - 10.1016/j.bios.2011.12.011

M3 - Article

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AN - SCOPUS:84855758931

SN - 0956-5663

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SP - 208

EP - 212

JO - Biosensors and Bioelectronics

JF - Biosensors and Bioelectronics

IS - 1

ER -

Aptamer-DNAzyme hairpins for biosensing of Ochratoxin A

Abstract

Keywords

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