Aptamer-DNAzyme hairpins for biosensing of Ochratoxin A

Cheng Yang, Vasilica Lates, Beatriz Prieto-Simón, Jean Louis Marty, Xiurong Yang

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We report an aptasensor for biosensing of Ochratoxin A (OTA) using aptamer-DNAzyme hairpin as biorecognition element. The structure of this engineered nucleic acid includes the horseradish peroxidase (HRP)-mimicking DNAzyme and the OTA specific aptamer sequences. A blocking tail captures a part of these sequences in the stem region of the hairpin. In the presence of OTA, the hairpin is opened due to the formation of the aptamer-analyte complex. As a result, self-assembly of the active HRP-mimicking DNAzyme occurs. The activity of this DNAzyme is linearly correlated with OTA concentration up to 10. nM, showing a limit of detection of 2.5. nM.

Original languageEnglish
Pages (from-to)208-212
Number of pages5
JournalBiosensors and Bioelectronics
Issue number1
Publication statusPublished - 15 Feb 2012
Externally publishedYes


  • Aptamer
  • DNAzyme
  • G-quadruplex
  • Hairpin
  • Ochratoxin A

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