Aptamer-DNAzyme hairpins for biosensing of Ochratoxin A

Cheng Yang, Vasilica Lates, Beatriz Prieto-Simón, Jean Louis Marty, Xiurong Yang

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110 Citations (Scopus)

Abstract

We report an aptasensor for biosensing of Ochratoxin A (OTA) using aptamer-DNAzyme hairpin as biorecognition element. The structure of this engineered nucleic acid includes the horseradish peroxidase (HRP)-mimicking DNAzyme and the OTA specific aptamer sequences. A blocking tail captures a part of these sequences in the stem region of the hairpin. In the presence of OTA, the hairpin is opened due to the formation of the aptamer-analyte complex. As a result, self-assembly of the active HRP-mimicking DNAzyme occurs. The activity of this DNAzyme is linearly correlated with OTA concentration up to 10. nM, showing a limit of detection of 2.5. nM.

Original languageEnglish
Pages (from-to)208-212
Number of pages5
JournalBiosensors and Bioelectronics
Volume32
Issue number1
DOIs
Publication statusPublished - 15 Feb 2012
Externally publishedYes

Keywords

  • Aptamer
  • DNAzyme
  • G-quadruplex
  • Hairpin
  • Ochratoxin A

Cite this

Yang, C., Lates, V., Prieto-Simón, B., Marty, J. L., & Yang, X. (2012). Aptamer-DNAzyme hairpins for biosensing of Ochratoxin A. Biosensors and Bioelectronics, 32(1), 208-212. https://doi.org/10.1016/j.bios.2011.12.011