Application of specific ELISAs for BMP15 and GDF9 to cumulus cell extracts from infertile women

A. E. Krysta-Matter; A. H. Riepsamen; S. Lien; W. Y.T. Wong; D. Richani; S. Kilani; C. A. Harrison; K. A. Mallitt; W. L. Ledger; D. M. Robertson; R. B. Gilchrist

doi:10.1016/j.mce.2023.112049

Application of specific ELISAs for BMP15 and GDF9 to cumulus cell extracts from infertile women

A. E. Krysta-Matter, A. H. Riepsamen, S. Lien, W. Y.T. Wong, D. Richani, S. Kilani, C. A. Harrison, K. A. Mallitt, W. L. Ledger, D. M. Robertson, R. B. Gilchrist

Research output: Contribution to journal › Article › Research › peer-review

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Abstract

Bone morphogenetic protein 15 (BMP15) and growth differentiation factor 9 (GDF9) are oocyte-specific paracrine factors which regulate ovarian cumulus cell (CC) functions. This study aimed to investigate if BMP15 and GDF9 bound to CCs can be characterized, quantified, and show an association with IVF outcomes in infertile women. BMP15 and GDF9 ELISAs were validated and applied to discarded CC extracts. Pooled CCs from individual patients were collected from 120 (cohort 1; BMP15 only) and 81 infertility patients (cohort 2; BMP15 and GDF9) undergoing superovulation. BMP15 and GDF9 levels expressed per CC DNA were correlated with maternal age, clinical and embryology data. Total BMP15 and GDF9 were highly correlated with each other (r = 0.9, p < 0.001). The GDF9:BMP15 ratio was unrelated to oocyte number or age. BMP15/CC DNA and GDF9/CC DNA were unaffected by the type of superovulation and were not related to oocyte/embryo outcomes.

Original language	English
Article number	112049
Number of pages	11
Journal	Molecular and Cellular Endocrinology
Volume	578
DOIs	https://doi.org/10.1016/j.mce.2023.112049
Publication status	Published - 1 Dec 2023

Keywords

Bone morphogenetic protein 15
Cumulin
Cumulus cells
Growth differentiating factor 9
Oocyte

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10.1016/j.mce.2023.112049Licence: CC BY

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Krysta-Matter, A. E., Riepsamen, A. H., Lien, S., Wong, W. Y. T., Richani, D., Kilani, S., Harrison, C. A., Mallitt, K. A., Ledger, W. L., Robertson, D. M., & Gilchrist, R. B. (2023). Application of specific ELISAs for BMP15 and GDF9 to cumulus cell extracts from infertile women. Molecular and Cellular Endocrinology, 578, Article 112049. https://doi.org/10.1016/j.mce.2023.112049

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title = "Application of specific ELISAs for BMP15 and GDF9 to cumulus cell extracts from infertile women",

abstract = "Bone morphogenetic protein 15 (BMP15) and growth differentiation factor 9 (GDF9) are oocyte-specific paracrine factors which regulate ovarian cumulus cell (CC) functions. This study aimed to investigate if BMP15 and GDF9 bound to CCs can be characterized, quantified, and show an association with IVF outcomes in infertile women. BMP15 and GDF9 ELISAs were validated and applied to discarded CC extracts. Pooled CCs from individual patients were collected from 120 (cohort 1; BMP15 only) and 81 infertility patients (cohort 2; BMP15 and GDF9) undergoing superovulation. BMP15 and GDF9 levels expressed per CC DNA were correlated with maternal age, clinical and embryology data. Total BMP15 and GDF9 were highly correlated with each other (r = 0.9, p < 0.001). The GDF9:BMP15 ratio was unrelated to oocyte number or age. BMP15/CC DNA and GDF9/CC DNA were unaffected by the type of superovulation and were not related to oocyte/embryo outcomes.",

keywords = "Bone morphogenetic protein 15, Cumulin, Cumulus cells, Growth differentiating factor 9, Oocyte",

author = "Krysta-Matter, {A. E.} and Riepsamen, {A. H.} and S. Lien and Wong, {W. Y.T.} and D. Richani and S. Kilani and Harrison, {C. A.} and Mallitt, {K. A.} and Ledger, {W. L.} and Robertson, {D. M.} and Gilchrist, {R. B.}",

note = "Funding Information: Approval of this study was granted by the IVF Australia Human Research Ethics Committee (R&D-109). This was a retrospective study with samples collected at a single centre at IVF Australia, Sydney, Australia.The study was funded by a grant (APP1121504) awarded to RBG, CAH, DMR and WLL and fellowships (APP1023210, APP1117538) awarded to RBG from the National Health and Medical Research Council of Australia. AEKM was a recipient of a Virtus Health sponsored PhD Scholarship in Reproductive Science. Funding Information: The study was funded by a grant ( APP1121504 ) awarded to RBG, CAH, DMR and WLL and fellowships ( APP1023210 , APP1117538 ) awarded to RBG from the National Health and Medical Research Council of Australia . AEKM was a recipient of a Virtus Health sponsored PhD Scholarship in Reproductive Science. Publisher Copyright: {\textcopyright} 2023 The Authors",

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doi = "10.1016/j.mce.2023.112049",

language = "English",

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AU - Riepsamen, A. H.

AU - Lien, S.

AU - Wong, W. Y.T.

AU - Richani, D.

AU - Kilani, S.

AU - Harrison, C. A.

AU - Mallitt, K. A.

AU - Ledger, W. L.

AU - Robertson, D. M.

AU - Gilchrist, R. B.

N1 - Funding Information: Approval of this study was granted by the IVF Australia Human Research Ethics Committee (R&D-109). This was a retrospective study with samples collected at a single centre at IVF Australia, Sydney, Australia.The study was funded by a grant (APP1121504) awarded to RBG, CAH, DMR and WLL and fellowships (APP1023210, APP1117538) awarded to RBG from the National Health and Medical Research Council of Australia. AEKM was a recipient of a Virtus Health sponsored PhD Scholarship in Reproductive Science. Funding Information: The study was funded by a grant ( APP1121504 ) awarded to RBG, CAH, DMR and WLL and fellowships ( APP1023210 , APP1117538 ) awarded to RBG from the National Health and Medical Research Council of Australia . AEKM was a recipient of a Virtus Health sponsored PhD Scholarship in Reproductive Science. Publisher Copyright: © 2023 The Authors

PY - 2023/12/1

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N2 - Bone morphogenetic protein 15 (BMP15) and growth differentiation factor 9 (GDF9) are oocyte-specific paracrine factors which regulate ovarian cumulus cell (CC) functions. This study aimed to investigate if BMP15 and GDF9 bound to CCs can be characterized, quantified, and show an association with IVF outcomes in infertile women. BMP15 and GDF9 ELISAs were validated and applied to discarded CC extracts. Pooled CCs from individual patients were collected from 120 (cohort 1; BMP15 only) and 81 infertility patients (cohort 2; BMP15 and GDF9) undergoing superovulation. BMP15 and GDF9 levels expressed per CC DNA were correlated with maternal age, clinical and embryology data. Total BMP15 and GDF9 were highly correlated with each other (r = 0.9, p < 0.001). The GDF9:BMP15 ratio was unrelated to oocyte number or age. BMP15/CC DNA and GDF9/CC DNA were unaffected by the type of superovulation and were not related to oocyte/embryo outcomes.

AB - Bone morphogenetic protein 15 (BMP15) and growth differentiation factor 9 (GDF9) are oocyte-specific paracrine factors which regulate ovarian cumulus cell (CC) functions. This study aimed to investigate if BMP15 and GDF9 bound to CCs can be characterized, quantified, and show an association with IVF outcomes in infertile women. BMP15 and GDF9 ELISAs were validated and applied to discarded CC extracts. Pooled CCs from individual patients were collected from 120 (cohort 1; BMP15 only) and 81 infertility patients (cohort 2; BMP15 and GDF9) undergoing superovulation. BMP15 and GDF9 levels expressed per CC DNA were correlated with maternal age, clinical and embryology data. Total BMP15 and GDF9 were highly correlated with each other (r = 0.9, p < 0.001). The GDF9:BMP15 ratio was unrelated to oocyte number or age. BMP15/CC DNA and GDF9/CC DNA were unaffected by the type of superovulation and were not related to oocyte/embryo outcomes.

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KW - Cumulin

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KW - Growth differentiating factor 9

KW - Oocyte

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DO - 10.1016/j.mce.2023.112049

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SN - 0303-7207

VL - 578

JO - Molecular and Cellular Endocrinology

JF - Molecular and Cellular Endocrinology

M1 - 112049

ER -

Application of specific ELISAs for BMP15 and GDF9 to cumulus cell extracts from infertile women

Abstract

Keywords

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Therapeutic potential of TGF-beta proteins for the diagnosis and treatment of female infertility

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Application of specific ELISAs for BMP15 and GDF9 to cumulus cell extracts from infertile women

Abstract

Keywords

Access to Document

Other files and links

Projects

Therapeutic potential of TGF-beta proteins for the diagnosis and treatment of female infertility

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