The application of pulsed electric field (PEF) as one of the nonthermal intensification methods has been proposed to increase oil yield extraction from sunflower seeds. PEF treatments have been carried out under various electrical parameters such as electric field, pulsed frequency, pulse width, time of treatment and sample conductivity. 55 fractional factorial design has been used for the analysis of the impact of PEF parameters on oil yield, and oil yield square model parameters have been investigated. The oil yield was found to increase by 9.1% after treatment of sunflower seeds by variation of PEF parameters: 30 s under an electric field of 7.0 kV/cm having frequency of 15 Hz, solvent content of 40 wt% and pulse width of 30 μs. Practical Applications: Here, we study the application of assisted pulsed electric field for oil extraction from sunflower seeds and we find the most effective variation of pulsed electric field parameters for treatment. It is very important in the industrial world as it deals with the time, quality of production and cost, which are usually carried out without preliminary treatment.