TY - JOUR
T1 - Application of natural rubber latex as scaffold for osteoblast to guided bone regeneration
AU - Borges, Felipe Azevedo
AU - de Barros, Natan Roberto
AU - Garms, Bruna Cambraia
AU - Miranda, Matheus Carlos Romeiro
AU - Gemeinder, Jose Lucio Padua
AU - Ribeiro-Paes, João Tadeu
AU - Silva, Rodrigo Ferreira
AU - de Toledo, Karina Alves
AU - Herculano, Rondinelli Donizetti
N1 - Funding Information:
The authors acknowledge the support of FAPESP (process number: 11/17411–8 and 14/17526–8) and CAPES.
Publisher Copyright:
© 2017 Wiley Periodicals, Inc.
PY - 2017/10/15
Y1 - 2017/10/15
N2 - Natural rubber latex (NRL) from Hevea brasiliensis is a colloidal system composed of cis-1,4-polyisoprene. Its applications have grown due its angiogenic and wound healing activity. NRL has been used in guided bone regeneration as barrier, enhancing bone formation. However, there has been no study reported so far which shows its in vitro biocompatibility with osteoblasts. Thus, the aim of this work was to apply thermally induced phase separation under several temperatures to induce porosity in NRL; and test its mechanical properties, cytotoxicity, cell adhesion, and mineralization with MC3T3-E1. Only biomembranes submitted at −20 and −10 °C presented porosity. Fourier transform infrared spectroscopy showed no change in cis-1,4-isoprene spectra. Biomembranes were elastic (Young's modulus < 1 MPa). According to ISO10993–5, NRL showed no cytotoxicity. Cells adhered on the NRL and produced mineral matrix as analyzed by scanning electron microscopy–energy-dispersive spectrometry, von kossa, and Fourier transform infrared spectroscopy. Cells on NRL presented higher alkaline phosphatase activity, however, mineralization showed no difference by alizarin red S dye extraction.
AB - Natural rubber latex (NRL) from Hevea brasiliensis is a colloidal system composed of cis-1,4-polyisoprene. Its applications have grown due its angiogenic and wound healing activity. NRL has been used in guided bone regeneration as barrier, enhancing bone formation. However, there has been no study reported so far which shows its in vitro biocompatibility with osteoblasts. Thus, the aim of this work was to apply thermally induced phase separation under several temperatures to induce porosity in NRL; and test its mechanical properties, cytotoxicity, cell adhesion, and mineralization with MC3T3-E1. Only biomembranes submitted at −20 and −10 °C presented porosity. Fourier transform infrared spectroscopy showed no change in cis-1,4-isoprene spectra. Biomembranes were elastic (Young's modulus < 1 MPa). According to ISO10993–5, NRL showed no cytotoxicity. Cells adhered on the NRL and produced mineral matrix as analyzed by scanning electron microscopy–energy-dispersive spectrometry, von kossa, and Fourier transform infrared spectroscopy. Cells on NRL presented higher alkaline phosphatase activity, however, mineralization showed no difference by alizarin red S dye extraction.
KW - biocompatibility
KW - bioengineering
KW - membranes
KW - porous materials
KW - rubber
UR - http://www.scopus.com/inward/record.url?scp=85020422098&partnerID=8YFLogxK
U2 - 10.1002/app.45321
DO - 10.1002/app.45321
M3 - Article
AN - SCOPUS:85020422098
SN - 0021-8995
VL - 134
JO - Journal of Applied Polymer Science
JF - Journal of Applied Polymer Science
IS - 39
M1 - 45321
ER -