Application of nanostructured biochips for efficient cell transfection microarrays

Yamini Akkamsetty, Andrew L. Hook, Helmut Thissen, Jason P. Hayes, Nicolas H. Voelcker

Research output: Chapter in Book/Report/Conference proceedingConference PaperOtherpeer-review

1 Citation (Scopus)

Abstract

Microarrays, high-throughput devices for genomic analysis, can be further improved by developing materials that are able to manipulate the interfacial behaviour of biomolecules. This is achieved both spatially and temporally by smart materials possessing both switchable and patterned surface properties. A system had been developed to spatially manipulate both DNA and cell growth based upon the surface modification of highly doped silicon by ' plasma polymerisation and polyethylene grafting followed by masked laser ablation for formation of a pattered surface with both bioactive and non-fouling regions. This platform has been successfully applied to transfected cell microarray applications with the parallel expression of genes by utilising its ability to direct and limit both DNA and cell attachment to specific sites. One of the greatest advantages of this system is its application to reverse transfection, whereupon by utilising the switchable adsorption and desorption of DNA using a voltage bias, the efficiency of cell transfection can be enhanced. However, it was shown that application of a voltage also reduces the viability of neuroblastoma cells grown on a plasma polymer surface, but not human embryonic kidney cells. This suggests that the application of a voltage may not only result in the desorption of bound DNA but may also affect attached cells. The characterisation of a DNA microarray by contact printing has also been investigated.

Original languageEnglish
Title of host publicationSmart Materials IV
DOIs
Publication statusPublished - 27 Apr 2007
Externally publishedYes
EventSPIE International Symposium on Smart Materials, Nano- and Micro-Smart Systems - Adelaide, Australia
Duration: 11 Dec 200613 Dec 2006

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
Volume6413
ISSN (Print)0277-786X

Conference

ConferenceSPIE International Symposium on Smart Materials, Nano- and Micro-Smart Systems
CountryAustralia
CityAdelaide
Period11/12/0613/12/06

Keywords

  • Cell viability
  • Contact printing
  • Plasma polymerisation
  • Transfection cell microarray
  • Transfection efficiency

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