TY - JOUR
T1 - Application of HPTLC for simultaneous estimation of ambroxol hydrochloride and roxithromycin in pharmaceutical dosage forms
AU - Jaiswal, Yogini
AU - Shaikh, Mohd Farooq
AU - Kenjale, Rakesh
AU - Talele, Gokul
PY - 2012/12
Y1 - 2012/12
N2 - A simple, rapid and reliable HPTLC method was developed for the determination of Ambroxol Hydrochloride and Roxithromycin in tablets. Stress testing and stability studies were carried out for both the drugs as per the ICH guidelines. Determinations were performed on aluminium backed silica gel 60 F254 HPTLC plates, with conc. Ammonia, 1-propanol,ethylacetate, hexane 0.5+20+20+70(v/v), as mobile phase. The validated calibration range was 300ng - 1500ng spot-1 (r = 0.998) and 750 ng - 3750 ng (r = 0.997) for Ambroxol Hydrochloride and Roxithromycin respectively. The spots were scanned at λ = 254nm. The suitability of this HPTLC method for quantitative determination of compounds was proved by various validation parameters in accordance with the requirements of ICH guidelines (Q2B). Results from the stress testing studies indicated in the present research paper will form an integral part the development of new pharmaceutical formulations of Ambroxol hydrochloride and Roxithromycin and their estimation in biological fluids.
AB - A simple, rapid and reliable HPTLC method was developed for the determination of Ambroxol Hydrochloride and Roxithromycin in tablets. Stress testing and stability studies were carried out for both the drugs as per the ICH guidelines. Determinations were performed on aluminium backed silica gel 60 F254 HPTLC plates, with conc. Ammonia, 1-propanol,ethylacetate, hexane 0.5+20+20+70(v/v), as mobile phase. The validated calibration range was 300ng - 1500ng spot-1 (r = 0.998) and 750 ng - 3750 ng (r = 0.997) for Ambroxol Hydrochloride and Roxithromycin respectively. The spots were scanned at λ = 254nm. The suitability of this HPTLC method for quantitative determination of compounds was proved by various validation parameters in accordance with the requirements of ICH guidelines (Q2B). Results from the stress testing studies indicated in the present research paper will form an integral part the development of new pharmaceutical formulations of Ambroxol hydrochloride and Roxithromycin and their estimation in biological fluids.
KW - Ambroxol hydrochloride
KW - HPTLC
KW - Pharmaceutical dosage forms
KW - Roxithromycin
UR - http://www.scopus.com/inward/record.url?scp=84893086376&partnerID=8YFLogxK
UR - http://www.eurasianjournals.com/pdf-79639-15698?filename=Application%20of%20HPTLC%20for.pdf
M3 - Article
AN - SCOPUS:84893086376
SN - 1306-3057
VL - 7
SP - 168
EP - 179
JO - Eurasian Journal of Analytical Chemistry
JF - Eurasian Journal of Analytical Chemistry
IS - 3
ER -