Application of comprehensive two-dimensional gas chromatography (GC × GC) to the enantioselective analysis of essential oils

Robert Shellie, Philip Marriott, Charles Cornwall

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A new method for the enantioselective analysis of essential oils is described, using comprehensive two-dimensional gas chromatography (GC × GC). The column set comprised a primary column containing a cyclodextrin derivative as a chiral selector, and a secondary column containing a polyethylene glycol stationary phase. A cryogenic modulation system was used to achieve the GC × GC experiment. The enantiomeric compositions of a number of monoterpene hydrocarbons and oxygenated monoterpenes in Australian tea tree (Melaleuca alternifolia), including α-thujene, sabinene, β-pinene, α-phellandrene, limonene, trans-sabinene hydrate, cis-sabinene hydrate, linalool, terpinen-4-ol, and α-terpineol are reported. The GC × GC resolution advantage is shown to improve the efficiency of enantioselective essential oil analyses. In a single temperature programmed analysis, the individual antipodes of optically active components can be separated, and are effectively free from matrix interferences.

Original languageEnglish
Pages (from-to)823-830
Number of pages8
JournalJournal of Separation Science
Issue number10-11
Publication statusPublished - 27 Dec 2001
Externally publishedYes


  • Comprehensive gas chromatography
  • Cryogenic modulation
  • Enantioselective GC
  • Melaleuca alternifolia
  • Monoterpene hydrocarbons
  • Oxygenated monoterpenes

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