Application of a novel diffraction-based tomography method for imaging biological samples

Chaminda Rajeev Samarage, Gregory Sheard, Andreas Fouras

Research output: Chapter in Book/Report/Conference proceedingConference PaperResearchpeer-review


A novel system to image and reconstruct a 3-dimensional map of the refractive index based on the diffraction of light through a transparent sample is presented. This method is tested and validated on computer-generated data sets. The proposed system is an advanced variation of an imaging technique used in engineering for the study of aerodynamics. This method, which is termed Reference Image Topography, is used to reconstruct the water/air interface of the free surface in fluid dynamics studies. This surface profile is reconstructed by comparing an image of a random pattern viewed through the transparent free surface against a reference image, to determine the change in the refractive index caused by changes in the height. The proposed system is highly sensitive and capable of imaging intricate features in the transparent sample that are of low contrast when imaged with other imaging methods. For each projection, the change in direction of the light passing through the sample when placed in between the light source and the imaging system, can be related to the line integral for the change in refractive index across the sample. Utilizing multiple projections, a 3- dimensional map of the refractive index of the sample is reconstructed with computed tomography.? (2012) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE).
Original languageEnglish
Title of host publicationProceedings of SPIE Volume 8506 - Developments in X-ray tomography VIII
EditorsStuart R Stock
Place of PublicationBellingham Washington USA
Pages1 - 9
Number of pages9
ISBN (Print)9780819492234
Publication statusPublished - 2012
EventSPIE Optics and Photonics 2012 - San Diego, United States of America
Duration: 1 Jan 2012 → …


ConferenceSPIE Optics and Photonics 2012
Country/TerritoryUnited States of America
CitySan Diego
Period1/01/12 → …

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