Appendix F: Quinn’s advantage embryo freeze kit

Kiri Beilby, Patrick Quinn

Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Otherpeer-review

Abstract

Despite a large focus on the use of vitrification to cryopreserve embryos in recent years, there are still arguments for the use of slow freezing for the cleavage-stage embryo. Having said this, there are lessons to be learned from the process of vitrification that could be applied to slow freezing to improve post-thaw survival and ultimately clinical pregnancy rates. Specifically, increasing the concentration of sucrose in the freezing solution from 0.1 to 0.2 M and subsequently increasing the sucrose concentrations in thawing solutions could prove beneficial. The use of vitrification warming solutions in the thawing of slow-frozen embryos may also be an option that not only improves survival but also streamlines product purchasing and protocols within the laboratory.

Original languageEnglish
Title of host publicationCryopreservation of Mammalian Gametes and Embryos
Subtitle of host publicationMethods in Molecular Biology
EditorsZsolt Peter Nagy, Alex C. Varghese, Ashok Agarwal
Place of PublicationNew York, NY
PublisherHumana Press
Pages343-354
Number of pages12
ISBN (Electronic)9781493968282
ISBN (Print)9781493968268
DOIs
Publication statusPublished - 2017

Publication series

NameMethods in Molecular Biology
PublisherHumana Press
Volume1568
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Cryopreservation
  • Cryoprotectants
  • Embryo
  • Slow cooling
  • Thawing

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