Abstract
| Original language | English |
|---|---|
| Article number | e1651 |
| Number of pages | 12 |
| Journal | Cell Death and Disease |
| Volume | 6 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 1 Jan 2015 |
Cite this
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APP intracellular domain acts as a transcriptional regulator of miR-663 suppressing neuronal differentiation. / Shu, Runzhe; Wong, W S Fred; Ma, Quan-Hong; Yang, Z Z; Zhu, H; Liu, F J; Wang, Ping; Ma, Jun; Yan, S; Polo, Jose Maria; Bernard, Claude Charles Andre; Stanton, Lawrence W; Dawe, Gavin S; Xiao, Zhi-cheng.
In: Cell Death and Disease, Vol. 6, No. 2, e1651, 01.01.2015.Research output: Contribution to journal › Article › Research › peer-review
TY - JOUR
T1 - APP intracellular domain acts as a transcriptional regulator of miR-663 suppressing neuronal differentiation
AU - Shu, Runzhe
AU - Wong, W S Fred
AU - Ma, Quan-Hong
AU - Yang, Z Z
AU - Zhu, H
AU - Liu, F J
AU - Wang, Ping
AU - Ma, Jun
AU - Yan, S
AU - Polo, Jose Maria
AU - Bernard, Claude Charles Andre
AU - Stanton, Lawrence W
AU - Dawe, Gavin S
AU - Xiao, Zhi-cheng
PY - 2015/1/1
Y1 - 2015/1/1
N2 - Amyloid precursor protein (APP) is best known for its involvement in the pathogenesis of Alzheimer s disease. We have previously demonstrated that APP intracellular domain (AICD) regulates neurogenesis; however, the mechanisms underlying AICD-mediated regulation of neuronal differentiation are not yet fully characterized. Using genome-wide chromatin immunoprecipitation approaches, we found that AICD is specifically recruited to the regulatory regions of several microRNA genes, and acts as a transcriptional regulator for miR-663, miR-3648 and miR-3687 in human neural stem cells. Functional assays show that AICD negatively modulates neuronal differentiation through miR-663, a primate-specific microRNA. Microarray data further demonstrate that miR-663 suppresses the expression of multiple genes implicated in neurogenesis, including FBXL18 and CDK6. Our results indicate that AICD has a novel role in suppression of neuronal differentiation via transcriptional regulation of miR-663 in human neural stem cells.
AB - Amyloid precursor protein (APP) is best known for its involvement in the pathogenesis of Alzheimer s disease. We have previously demonstrated that APP intracellular domain (AICD) regulates neurogenesis; however, the mechanisms underlying AICD-mediated regulation of neuronal differentiation are not yet fully characterized. Using genome-wide chromatin immunoprecipitation approaches, we found that AICD is specifically recruited to the regulatory regions of several microRNA genes, and acts as a transcriptional regulator for miR-663, miR-3648 and miR-3687 in human neural stem cells. Functional assays show that AICD negatively modulates neuronal differentiation through miR-663, a primate-specific microRNA. Microarray data further demonstrate that miR-663 suppresses the expression of multiple genes implicated in neurogenesis, including FBXL18 and CDK6. Our results indicate that AICD has a novel role in suppression of neuronal differentiation via transcriptional regulation of miR-663 in human neural stem cells.
UR - http://www.scopus.com/inward/record.url?scp=84927618580&partnerID=8YFLogxK
U2 - 10.1038/cddis.2015.10
DO - 10.1038/cddis.2015.10
M3 - Article
VL - 6
JO - Cell Death and Disease
JF - Cell Death and Disease
SN - 2041-4889
IS - 2
M1 - e1651
ER -