APELIN promotes hematopoiesis from human embryonic stem cells

Qing Cissy Yu, Claire E Hirst, Magdaline Costa, Elizabeth Siew Sun Ng, Jacqueline V Schiesser, Karin AM Gertow, Edouard G Stanley, Andrew G Elefanty

    Research output: Contribution to journalArticleResearchpeer-review

    38 Citations (Scopus)

    Abstract

    Transcriptional profiling of differentiating human embryonic stem cells (hESCs) revealed that MIXL1-positive mesodermal precursors were enriched for transcripts encoding the G-protein coupled APELIN receptor (APLNR). APLNR-positive cells, identified by binding of the fluoresceinated peptide ligand, APELIN (APLN), or an anti-APLNR monoclonal antibody, were found in both posterior mesoderm and anterior mesendoderm populations and were enriched in hemangioblast colony forming cells (Bl-CFC). The addition of APLN peptide to the media enhanced the growth of embryoid bodies (EBs), increased the expression of hematoendothelial genes in differentiating hESCs and increased the frequency of Bl-CFCs by up to 10 fold. Furthermore, APLN peptide also synergised with VEGF to promote the growth of hESC-derived endothelial cells. These studies identified APLN as a novel growth factor for hESC derived hematopoietic and endothelial cells.
    Original languageEnglish
    Pages (from-to)6243 - 6254
    Number of pages12
    JournalBlood
    Volume119
    Issue number26
    DOIs
    Publication statusPublished - 2012

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