Antiviral activity of arbidol, a broad-spectrum drug for use against respiratory viruses, varies according to test conditions

Megan Jade Brooks, Elena Burtseva, Philip J Ellery, Glenn A Marsh, Andrew M Lew, Anatoly Slepushkin, Suzanne Crowe, Gregory A Tannock

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49 Citations (Scopus)

Abstract

The therapeutic activity of arbidol was investigated against representatives of seven different virus families. Its 50 median effective concentration (EC 50) was 0.22-11.8?g/ml (0.41-22nM). Therapeutic indices of 91 were obtained for type 1 poliovirus and 1.9-8.5 for influenza A and B, human paramyxo-3, avian infectious bronchitis-, and Marek s disease viruses. Arbidol was more inhibitory for influenza A/Aichi/2/68 (H3N2) virus than rimantadine or amantadine (EC 50 10 vs. >15 and >31.6?g/ml); greater inhibition occurred when end-points were expressed as TCID 50s. For respiratory syncytial virus (RSV), a reduction in plaque size but not number was observed. However, when the drug was added to infected cultures (=5?g/ml), a 3-log reduction in titer occurred. Arbidol did not inhibit directly influenza A/Aichi/2/68 hemagglutinin (HA) or neuraminidase (NA) activity, but inhibition of fusion between the viral envelope and chicken red blood cells occurred when added at 0.1?g/ml prior to infection. Arbidol induced changes to viral mRNA synthesis of the PB2, PA, NP, NA, and NS genes in MDCK cultures infected with influenza A/PR/8/34. There was no indirect evidence of enhancement of interferon-a by arbidol following infection with A/Aichi/2/68. Arbidol neither reduced lung viral titers nor caused a significant reduction of lung consolidation in BALB/c mice after administration by the oral and intraperitoneal (i.p.) routes and intranasal challenge with influenza A/Aichi/2/68. A small reduction in lung consolidation, but not viral titer, occurred after i.p. administration and subsequent challenge with RSV. The results indicate the potential of arbidol as a broad-spectrum respiratory antiviral drug.
Original languageEnglish
Pages (from-to)170 - 181
Number of pages12
JournalJournal of Medical Virology
Volume84
Issue number1
DOIs
Publication statusPublished - 2012

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