Antiseptic mouthwash against pharyngeal Neisseria gonorrhoeae:A randomised controlled trial and an in vitro study

Eric P F Chow, Benjamin P. Howden, Sandra Walker, David Lee, Catriona S. Bradshaw, Marcus Y. Chen, Anthony Snow, Stuart Cook, Glenda Fehler, Christopher K. Fairley

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35 Citations (Scopus)

Abstract

Background Gonorrhoea is increasing among men who have sex with men (MSM). We aimed to determine whether Listerine, a commercial mouthwash product, has an inhibitory effect against Neisseria gonorrhoeae in a randomised controlled trial (RCT) and an in vitro study, and therefore may be a potentially useful agent for gonorrhoea control. Methods In vitro: a suspension of ~108 colony forming units per mL (CFU/mL) of N. gonorrhoeae was added to a serial of dilutions (up to 1:32) of alcoholcontaining Listerine mouthwashes (Cool Mint and Total Care) for 1 min. A 10 mL aliquot was spread over the surface of a gonococcal agar plate and the number of N. gonorrhoeae colonies present at each dilution was calculated. The phosphate buffered saline (PBS) was used as a control. RCT: we recruited MSM with pharyngeal gonorrhoea who returned for treatment at the Melbourne Sexual Health Centre between May 2015 and February 2016. Untreated men were randomised to rinse and gargle either Listerine Cool Mint or saline for 1 min. Pharyngeal swabs were taken before and after rinsing and gargling for culture of N. gonorrhoeae. The analysis included only men who were culture positive for N. gonorrhoeae before using the allocated solution on the day of recruitment. Results In vitro: Listerine mouthwashes at dilutions of up to 1:4 for 1 min resulted in significant reduction of total N. gonorrhoeae counts but PBS has no inhibitory effect against N. gonorrhoeae. RCT: a total of 196 MSM were recruited, 58 (30%) were culture positive before using the solution. After gargling the allocated solution, men in the Listerine group were significantly less likely to be culture positive on the pharyngeal surface (52%) compared with men in the saline group (84%) (p=0.013). Conclusions This data suggest Listerine, significantly reduces the amount of N. gonorrhoeae on the pharyngeal surface. With daily use it may increase gonococcal clearance and have important implications for prevention strategies. Trial registration number ACTRN12615000716561.

Original languageEnglish
Pages (from-to)88-93
Number of pages6
JournalSexually Transmitted Infections
Volume93
Issue number2
DOIs
Publication statusPublished - 2017

Cite this

@article{e8e87e87be974149be31151dbe4e6678,
title = "Antiseptic mouthwash against pharyngeal Neisseria gonorrhoeae:A randomised controlled trial and an in vitro study",
abstract = "Background Gonorrhoea is increasing among men who have sex with men (MSM). We aimed to determine whether Listerine, a commercial mouthwash product, has an inhibitory effect against Neisseria gonorrhoeae in a randomised controlled trial (RCT) and an in vitro study, and therefore may be a potentially useful agent for gonorrhoea control. Methods In vitro: a suspension of ~108 colony forming units per mL (CFU/mL) of N. gonorrhoeae was added to a serial of dilutions (up to 1:32) of alcoholcontaining Listerine mouthwashes (Cool Mint and Total Care) for 1 min. A 10 mL aliquot was spread over the surface of a gonococcal agar plate and the number of N. gonorrhoeae colonies present at each dilution was calculated. The phosphate buffered saline (PBS) was used as a control. RCT: we recruited MSM with pharyngeal gonorrhoea who returned for treatment at the Melbourne Sexual Health Centre between May 2015 and February 2016. Untreated men were randomised to rinse and gargle either Listerine Cool Mint or saline for 1 min. Pharyngeal swabs were taken before and after rinsing and gargling for culture of N. gonorrhoeae. The analysis included only men who were culture positive for N. gonorrhoeae before using the allocated solution on the day of recruitment. Results In vitro: Listerine mouthwashes at dilutions of up to 1:4 for 1 min resulted in significant reduction of total N. gonorrhoeae counts but PBS has no inhibitory effect against N. gonorrhoeae. RCT: a total of 196 MSM were recruited, 58 (30{\%}) were culture positive before using the solution. After gargling the allocated solution, men in the Listerine group were significantly less likely to be culture positive on the pharyngeal surface (52{\%}) compared with men in the saline group (84{\%}) (p=0.013). Conclusions This data suggest Listerine, significantly reduces the amount of N. gonorrhoeae on the pharyngeal surface. With daily use it may increase gonococcal clearance and have important implications for prevention strategies. Trial registration number ACTRN12615000716561.",
author = "Chow, {Eric P F} and Howden, {Benjamin P.} and Sandra Walker and David Lee and Bradshaw, {Catriona S.} and Chen, {Marcus Y.} and Anthony Snow and Stuart Cook and Glenda Fehler and Fairley, {Christopher K.}",
year = "2017",
doi = "10.1136/sextrans-2016-052753",
language = "English",
volume = "93",
pages = "88--93",
journal = "Sexually Transmitted Infections",
issn = "1368-4973",
publisher = "BMJ Group",
number = "2",

}

Antiseptic mouthwash against pharyngeal Neisseria gonorrhoeae:A randomised controlled trial and an in vitro study. / Chow, Eric P F; Howden, Benjamin P.; Walker, Sandra; Lee, David; Bradshaw, Catriona S.; Chen, Marcus Y.; Snow, Anthony; Cook, Stuart; Fehler, Glenda; Fairley, Christopher K.

In: Sexually Transmitted Infections, Vol. 93, No. 2, 2017, p. 88-93.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Antiseptic mouthwash against pharyngeal Neisseria gonorrhoeae:A randomised controlled trial and an in vitro study

AU - Chow, Eric P F

AU - Howden, Benjamin P.

AU - Walker, Sandra

AU - Lee, David

AU - Bradshaw, Catriona S.

AU - Chen, Marcus Y.

AU - Snow, Anthony

AU - Cook, Stuart

AU - Fehler, Glenda

AU - Fairley, Christopher K.

PY - 2017

Y1 - 2017

N2 - Background Gonorrhoea is increasing among men who have sex with men (MSM). We aimed to determine whether Listerine, a commercial mouthwash product, has an inhibitory effect against Neisseria gonorrhoeae in a randomised controlled trial (RCT) and an in vitro study, and therefore may be a potentially useful agent for gonorrhoea control. Methods In vitro: a suspension of ~108 colony forming units per mL (CFU/mL) of N. gonorrhoeae was added to a serial of dilutions (up to 1:32) of alcoholcontaining Listerine mouthwashes (Cool Mint and Total Care) for 1 min. A 10 mL aliquot was spread over the surface of a gonococcal agar plate and the number of N. gonorrhoeae colonies present at each dilution was calculated. The phosphate buffered saline (PBS) was used as a control. RCT: we recruited MSM with pharyngeal gonorrhoea who returned for treatment at the Melbourne Sexual Health Centre between May 2015 and February 2016. Untreated men were randomised to rinse and gargle either Listerine Cool Mint or saline for 1 min. Pharyngeal swabs were taken before and after rinsing and gargling for culture of N. gonorrhoeae. The analysis included only men who were culture positive for N. gonorrhoeae before using the allocated solution on the day of recruitment. Results In vitro: Listerine mouthwashes at dilutions of up to 1:4 for 1 min resulted in significant reduction of total N. gonorrhoeae counts but PBS has no inhibitory effect against N. gonorrhoeae. RCT: a total of 196 MSM were recruited, 58 (30%) were culture positive before using the solution. After gargling the allocated solution, men in the Listerine group were significantly less likely to be culture positive on the pharyngeal surface (52%) compared with men in the saline group (84%) (p=0.013). Conclusions This data suggest Listerine, significantly reduces the amount of N. gonorrhoeae on the pharyngeal surface. With daily use it may increase gonococcal clearance and have important implications for prevention strategies. Trial registration number ACTRN12615000716561.

AB - Background Gonorrhoea is increasing among men who have sex with men (MSM). We aimed to determine whether Listerine, a commercial mouthwash product, has an inhibitory effect against Neisseria gonorrhoeae in a randomised controlled trial (RCT) and an in vitro study, and therefore may be a potentially useful agent for gonorrhoea control. Methods In vitro: a suspension of ~108 colony forming units per mL (CFU/mL) of N. gonorrhoeae was added to a serial of dilutions (up to 1:32) of alcoholcontaining Listerine mouthwashes (Cool Mint and Total Care) for 1 min. A 10 mL aliquot was spread over the surface of a gonococcal agar plate and the number of N. gonorrhoeae colonies present at each dilution was calculated. The phosphate buffered saline (PBS) was used as a control. RCT: we recruited MSM with pharyngeal gonorrhoea who returned for treatment at the Melbourne Sexual Health Centre between May 2015 and February 2016. Untreated men were randomised to rinse and gargle either Listerine Cool Mint or saline for 1 min. Pharyngeal swabs were taken before and after rinsing and gargling for culture of N. gonorrhoeae. The analysis included only men who were culture positive for N. gonorrhoeae before using the allocated solution on the day of recruitment. Results In vitro: Listerine mouthwashes at dilutions of up to 1:4 for 1 min resulted in significant reduction of total N. gonorrhoeae counts but PBS has no inhibitory effect against N. gonorrhoeae. RCT: a total of 196 MSM were recruited, 58 (30%) were culture positive before using the solution. After gargling the allocated solution, men in the Listerine group were significantly less likely to be culture positive on the pharyngeal surface (52%) compared with men in the saline group (84%) (p=0.013). Conclusions This data suggest Listerine, significantly reduces the amount of N. gonorrhoeae on the pharyngeal surface. With daily use it may increase gonococcal clearance and have important implications for prevention strategies. Trial registration number ACTRN12615000716561.

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U2 - 10.1136/sextrans-2016-052753

DO - 10.1136/sextrans-2016-052753

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JO - Sexually Transmitted Infections

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