Antisenescence effect of mouse embryonic stem cell conditioned medium through a PDGF/FGF pathway

Yun Ui Bae, Joon Hyuk Choi, Andras Nagy, Hoon Ki Sung, Jae Ryong Kim

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Cellular senescence, an irreversible state of growth arrest, underlies organismal aging and agerelated diseases. Recent evidence suggests that aging intervention based on inhibition of cellular senescence might be a promising strategy for treatment of aging and age-related diseases. Embryonic stem cells (ESCs) and ESC conditioned medium (CM) have been suggested as a desirable source for regenerativemedicine.However, effects of ESC-CM on cellular senescence remain to be determined.We found that treatment of senescent human dermal fibroblasts with CM from mouse ESCs (mESCs) decreases senescence phenotypes. Wefound that plateletderived growth factor BB in mESC-CM plays a critical role in antisenescence effect of mESC-CM through upregulation of fibroblast growth factor 2. We confirmed that mESC-CM treatment accelerates the wound-healing process by down-regulating senescence-associated p53 expression in in vivo models. Taken together, our results suggest that mESC-CM has the ability to suppress cellular senescence and maintain proliferative capacity. Therefore, this strategy might emerge as a novel therapeutic strategy for aging and age-related diseases.

Original languageEnglish
Pages (from-to)1276-1286
Number of pages11
JournalFASEB Journal
Volume30
Issue number3
DOIs
Publication statusPublished - 1 Mar 2016
Externally publishedYes

Keywords

  • Cellular senescence
  • Conditioned medium
  • Embryonic stem cells
  • PDGF-BB

Cite this

Bae, Yun Ui ; Choi, Joon Hyuk ; Nagy, Andras ; Sung, Hoon Ki ; Kim, Jae Ryong. / Antisenescence effect of mouse embryonic stem cell conditioned medium through a PDGF/FGF pathway. In: FASEB Journal. 2016 ; Vol. 30, No. 3. pp. 1276-1286.
@article{ac65d3d8ac17419a8fdeb35f8f01ecf2,
title = "Antisenescence effect of mouse embryonic stem cell conditioned medium through a PDGF/FGF pathway",
abstract = "Cellular senescence, an irreversible state of growth arrest, underlies organismal aging and agerelated diseases. Recent evidence suggests that aging intervention based on inhibition of cellular senescence might be a promising strategy for treatment of aging and age-related diseases. Embryonic stem cells (ESCs) and ESC conditioned medium (CM) have been suggested as a desirable source for regenerativemedicine.However, effects of ESC-CM on cellular senescence remain to be determined.We found that treatment of senescent human dermal fibroblasts with CM from mouse ESCs (mESCs) decreases senescence phenotypes. Wefound that plateletderived growth factor BB in mESC-CM plays a critical role in antisenescence effect of mESC-CM through upregulation of fibroblast growth factor 2. We confirmed that mESC-CM treatment accelerates the wound-healing process by down-regulating senescence-associated p53 expression in in vivo models. Taken together, our results suggest that mESC-CM has the ability to suppress cellular senescence and maintain proliferative capacity. Therefore, this strategy might emerge as a novel therapeutic strategy for aging and age-related diseases.",
keywords = "Cellular senescence, Conditioned medium, Embryonic stem cells, PDGF-BB",
author = "Bae, {Yun Ui} and Choi, {Joon Hyuk} and Andras Nagy and Sung, {Hoon Ki} and Kim, {Jae Ryong}",
year = "2016",
month = "3",
day = "1",
doi = "10.1096/fj.15-278846",
language = "English",
volume = "30",
pages = "1276--1286",
journal = "FASEB Journal",
issn = "0892-6638",
publisher = "FASEB",
number = "3",

}

Antisenescence effect of mouse embryonic stem cell conditioned medium through a PDGF/FGF pathway. / Bae, Yun Ui; Choi, Joon Hyuk; Nagy, Andras; Sung, Hoon Ki; Kim, Jae Ryong.

In: FASEB Journal, Vol. 30, No. 3, 01.03.2016, p. 1276-1286.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Antisenescence effect of mouse embryonic stem cell conditioned medium through a PDGF/FGF pathway

AU - Bae, Yun Ui

AU - Choi, Joon Hyuk

AU - Nagy, Andras

AU - Sung, Hoon Ki

AU - Kim, Jae Ryong

PY - 2016/3/1

Y1 - 2016/3/1

N2 - Cellular senescence, an irreversible state of growth arrest, underlies organismal aging and agerelated diseases. Recent evidence suggests that aging intervention based on inhibition of cellular senescence might be a promising strategy for treatment of aging and age-related diseases. Embryonic stem cells (ESCs) and ESC conditioned medium (CM) have been suggested as a desirable source for regenerativemedicine.However, effects of ESC-CM on cellular senescence remain to be determined.We found that treatment of senescent human dermal fibroblasts with CM from mouse ESCs (mESCs) decreases senescence phenotypes. Wefound that plateletderived growth factor BB in mESC-CM plays a critical role in antisenescence effect of mESC-CM through upregulation of fibroblast growth factor 2. We confirmed that mESC-CM treatment accelerates the wound-healing process by down-regulating senescence-associated p53 expression in in vivo models. Taken together, our results suggest that mESC-CM has the ability to suppress cellular senescence and maintain proliferative capacity. Therefore, this strategy might emerge as a novel therapeutic strategy for aging and age-related diseases.

AB - Cellular senescence, an irreversible state of growth arrest, underlies organismal aging and agerelated diseases. Recent evidence suggests that aging intervention based on inhibition of cellular senescence might be a promising strategy for treatment of aging and age-related diseases. Embryonic stem cells (ESCs) and ESC conditioned medium (CM) have been suggested as a desirable source for regenerativemedicine.However, effects of ESC-CM on cellular senescence remain to be determined.We found that treatment of senescent human dermal fibroblasts with CM from mouse ESCs (mESCs) decreases senescence phenotypes. Wefound that plateletderived growth factor BB in mESC-CM plays a critical role in antisenescence effect of mESC-CM through upregulation of fibroblast growth factor 2. We confirmed that mESC-CM treatment accelerates the wound-healing process by down-regulating senescence-associated p53 expression in in vivo models. Taken together, our results suggest that mESC-CM has the ability to suppress cellular senescence and maintain proliferative capacity. Therefore, this strategy might emerge as a novel therapeutic strategy for aging and age-related diseases.

KW - Cellular senescence

KW - Conditioned medium

KW - Embryonic stem cells

KW - PDGF-BB

UR - http://www.scopus.com/inward/record.url?scp=84963852538&partnerID=8YFLogxK

U2 - 10.1096/fj.15-278846

DO - 10.1096/fj.15-278846

M3 - Article

VL - 30

SP - 1276

EP - 1286

JO - FASEB Journal

JF - FASEB Journal

SN - 0892-6638

IS - 3

ER -