Antisenescence effect of mouse embryonic stem cell conditioned medium through a PDGF/FGF pathway

Yun Ui Bae, Joon Hyuk Choi, Andras Nagy, Hoon Ki Sung, Jae Ryong Kim

Research output: Contribution to journalArticleResearchpeer-review

21 Citations (Scopus)

Abstract

Cellular senescence, an irreversible state of growth arrest, underlies organismal aging and agerelated diseases. Recent evidence suggests that aging intervention based on inhibition of cellular senescence might be a promising strategy for treatment of aging and age-related diseases. Embryonic stem cells (ESCs) and ESC conditioned medium (CM) have been suggested as a desirable source for regenerativemedicine.However, effects of ESC-CM on cellular senescence remain to be determined.We found that treatment of senescent human dermal fibroblasts with CM from mouse ESCs (mESCs) decreases senescence phenotypes. Wefound that plateletderived growth factor BB in mESC-CM plays a critical role in antisenescence effect of mESC-CM through upregulation of fibroblast growth factor 2. We confirmed that mESC-CM treatment accelerates the wound-healing process by down-regulating senescence-associated p53 expression in in vivo models. Taken together, our results suggest that mESC-CM has the ability to suppress cellular senescence and maintain proliferative capacity. Therefore, this strategy might emerge as a novel therapeutic strategy for aging and age-related diseases.

Original languageEnglish
Pages (from-to)1276-1286
Number of pages11
JournalThe FASEB Journal
Volume30
Issue number3
DOIs
Publication statusPublished - 1 Mar 2016
Externally publishedYes

Keywords

  • Cellular senescence
  • Conditioned medium
  • Embryonic stem cells
  • PDGF-BB

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