Antipyretic, Antinociceptive, and Anti-Inflammatory Activities from Pogostemon benghalensis Leaf Extract in Experimental Wister Rats

Sushant Aryal, Balkrishna Adhikari, Kasmira Panthi, Pramod Aryal, Shyam Kumar Mallik, Ram Prasad Bhusal, Bahare Salehi, William N. Setzer, Javad Sharifi-Rad, Niranjan Koirala

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Background: Pogostemon benghalensis leaves have traditionally been utilized for relieving body aches, headaches and fever. Based on its uses, the present study was designed to investigate the antinociceptive, antipyretic and anti-edematogenic activities from P. benghalensis leaves’ methanol extract (PBME) in Wister rats. Methods: The thermal (hot plate) and chemical (acetic acid-induced writhing and formalin test) models for antinociceptive effects, and the Brewer’s yeast induced hyperthermia test for antipyretic action and rat paw edema by carrageenan for anti-edematogenic activity, were applied for PBME at different dose levels. The acute toxicity of PBME through the oral route was performed to determine the lethal dose. Results: PBME significantly and dose-dependently reduced pyrexia and diminished edema volume, which depicted its antipyretic and anti-edematogenic effects respectively. The inhibition of writhing reflex, increased reaction latency and reduced frequency of licking indicated that PBME has significant dose-dependent antinociceptive activity. P. benghalensis methanol extract at 4000 mg/kg shows no sign of toxicity, which is a considerable, good margin of safety. Conclusions: The study illustrated the antipyretic, antinociceptive and anti-inflammatory potential of P. benghalensis leaf extract with a safety margin, and validated its traditional use to alleviate fever, pain, and inflammation.
Original languageEnglish
Article number96
Number of pages11
JournalMedicines
Volume6
Issue number4
DOIs
Publication statusPublished - 20 Sep 2019

Keywords

  • Pogostemon benghalensis
  • antipyretic
  • antinociceptive
  • anti-inflammatory
  • PBME
  • methanolic extract

Cite this

Aryal, Sushant ; Adhikari, Balkrishna ; Panthi, Kasmira ; Aryal, Pramod ; Mallik, Shyam Kumar ; Bhusal, Ram Prasad ; Salehi, Bahare ; Setzer, William N. ; Sharifi-Rad, Javad ; Koirala, Niranjan. / Antipyretic, Antinociceptive, and Anti-Inflammatory Activities from Pogostemon benghalensis Leaf Extract in Experimental Wister Rats. In: Medicines. 2019 ; Vol. 6, No. 4.
@article{e6977c2b0e9c4e2ea0dcb136c18af84d,
title = "Antipyretic, Antinociceptive, and Anti-Inflammatory Activities from Pogostemon benghalensis Leaf Extract in Experimental Wister Rats",
abstract = "Background: Pogostemon benghalensis leaves have traditionally been utilized for relieving body aches, headaches and fever. Based on its uses, the present study was designed to investigate the antinociceptive, antipyretic and anti-edematogenic activities from P. benghalensis leaves’ methanol extract (PBME) in Wister rats. Methods: The thermal (hot plate) and chemical (acetic acid-induced writhing and formalin test) models for antinociceptive effects, and the Brewer’s yeast induced hyperthermia test for antipyretic action and rat paw edema by carrageenan for anti-edematogenic activity, were applied for PBME at different dose levels. The acute toxicity of PBME through the oral route was performed to determine the lethal dose. Results: PBME significantly and dose-dependently reduced pyrexia and diminished edema volume, which depicted its antipyretic and anti-edematogenic effects respectively. The inhibition of writhing reflex, increased reaction latency and reduced frequency of licking indicated that PBME has significant dose-dependent antinociceptive activity. P. benghalensis methanol extract at 4000 mg/kg shows no sign of toxicity, which is a considerable, good margin of safety. Conclusions: The study illustrated the antipyretic, antinociceptive and anti-inflammatory potential of P. benghalensis leaf extract with a safety margin, and validated its traditional use to alleviate fever, pain, and inflammation.",
keywords = "Pogostemon benghalensis, antipyretic, antinociceptive, anti-inflammatory, PBME, methanolic extract",
author = "Sushant Aryal and Balkrishna Adhikari and Kasmira Panthi and Pramod Aryal and Mallik, {Shyam Kumar} and Bhusal, {Ram Prasad} and Bahare Salehi and Setzer, {William N.} and Javad Sharifi-Rad and Niranjan Koirala",
year = "2019",
month = "9",
day = "20",
doi = "10.3390/medicines6040096",
language = "English",
volume = "6",
journal = "Medicines",
issn = "2305-6320",
publisher = "MDPI",
number = "4",

}

Aryal, S, Adhikari, B, Panthi, K, Aryal, P, Mallik, SK, Bhusal, RP, Salehi, B, Setzer, WN, Sharifi-Rad, J & Koirala, N 2019, 'Antipyretic, Antinociceptive, and Anti-Inflammatory Activities from Pogostemon benghalensis Leaf Extract in Experimental Wister Rats', Medicines, vol. 6, no. 4, 96. https://doi.org/10.3390/medicines6040096

Antipyretic, Antinociceptive, and Anti-Inflammatory Activities from Pogostemon benghalensis Leaf Extract in Experimental Wister Rats. / Aryal, Sushant; Adhikari, Balkrishna; Panthi, Kasmira; Aryal, Pramod; Mallik, Shyam Kumar; Bhusal, Ram Prasad; Salehi, Bahare; Setzer, William N.; Sharifi-Rad, Javad; Koirala, Niranjan.

In: Medicines, Vol. 6, No. 4, 96, 20.09.2019.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Antipyretic, Antinociceptive, and Anti-Inflammatory Activities from Pogostemon benghalensis Leaf Extract in Experimental Wister Rats

AU - Aryal, Sushant

AU - Adhikari, Balkrishna

AU - Panthi, Kasmira

AU - Aryal, Pramod

AU - Mallik, Shyam Kumar

AU - Bhusal, Ram Prasad

AU - Salehi, Bahare

AU - Setzer, William N.

AU - Sharifi-Rad, Javad

AU - Koirala, Niranjan

PY - 2019/9/20

Y1 - 2019/9/20

N2 - Background: Pogostemon benghalensis leaves have traditionally been utilized for relieving body aches, headaches and fever. Based on its uses, the present study was designed to investigate the antinociceptive, antipyretic and anti-edematogenic activities from P. benghalensis leaves’ methanol extract (PBME) in Wister rats. Methods: The thermal (hot plate) and chemical (acetic acid-induced writhing and formalin test) models for antinociceptive effects, and the Brewer’s yeast induced hyperthermia test for antipyretic action and rat paw edema by carrageenan for anti-edematogenic activity, were applied for PBME at different dose levels. The acute toxicity of PBME through the oral route was performed to determine the lethal dose. Results: PBME significantly and dose-dependently reduced pyrexia and diminished edema volume, which depicted its antipyretic and anti-edematogenic effects respectively. The inhibition of writhing reflex, increased reaction latency and reduced frequency of licking indicated that PBME has significant dose-dependent antinociceptive activity. P. benghalensis methanol extract at 4000 mg/kg shows no sign of toxicity, which is a considerable, good margin of safety. Conclusions: The study illustrated the antipyretic, antinociceptive and anti-inflammatory potential of P. benghalensis leaf extract with a safety margin, and validated its traditional use to alleviate fever, pain, and inflammation.

AB - Background: Pogostemon benghalensis leaves have traditionally been utilized for relieving body aches, headaches and fever. Based on its uses, the present study was designed to investigate the antinociceptive, antipyretic and anti-edematogenic activities from P. benghalensis leaves’ methanol extract (PBME) in Wister rats. Methods: The thermal (hot plate) and chemical (acetic acid-induced writhing and formalin test) models for antinociceptive effects, and the Brewer’s yeast induced hyperthermia test for antipyretic action and rat paw edema by carrageenan for anti-edematogenic activity, were applied for PBME at different dose levels. The acute toxicity of PBME through the oral route was performed to determine the lethal dose. Results: PBME significantly and dose-dependently reduced pyrexia and diminished edema volume, which depicted its antipyretic and anti-edematogenic effects respectively. The inhibition of writhing reflex, increased reaction latency and reduced frequency of licking indicated that PBME has significant dose-dependent antinociceptive activity. P. benghalensis methanol extract at 4000 mg/kg shows no sign of toxicity, which is a considerable, good margin of safety. Conclusions: The study illustrated the antipyretic, antinociceptive and anti-inflammatory potential of P. benghalensis leaf extract with a safety margin, and validated its traditional use to alleviate fever, pain, and inflammation.

KW - Pogostemon benghalensis

KW - antipyretic

KW - antinociceptive

KW - anti-inflammatory

KW - PBME

KW - methanolic extract

U2 - 10.3390/medicines6040096

DO - 10.3390/medicines6040096

M3 - Article

VL - 6

JO - Medicines

JF - Medicines

SN - 2305-6320

IS - 4

M1 - 96

ER -