TY - JOUR
T1 - Antioxidant properties and cardioprotective mechanism of Malaysian propolis in rats
AU - Ahmed, Romana
AU - Tanvir, E. M.
AU - Hossen, Md Sakib
AU - Afroz, Rizwana
AU - Ahmmed, Istiyak
AU - Rumpa, Nur E.Noushin
AU - Paul, Sudip
AU - Gan, Siew Hua
AU - Sulaiman, Siti Amrah
AU - Khalil, Md Ibrahim
N1 - Publisher Copyright:
Copyright © 2017 Romana Ahmed et al.
Copyright:
Copyright 2018 Elsevier B.V., All rights reserved.
PY - 2017/2/2
Y1 - 2017/2/2
N2 - Propolis contains high concentrations of polyphenols, flavonoids, tannins, ascorbic acid, and reducing sugars and proteins. Malaysian Propolis (MP) has been reported to exhibit high 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical-scavenging activity and ferric reducing antioxidant power (FRAP) values. Herein, we report the antioxidant properties and cardioprotective properties of MP in isoproterenol- (ISO-) inducedmyocardial infarction in rats.MaleWistar rats ( = 32) were pretreated orallywith an ethanol extract ofMP (100mg/kg/day) for 30 consecutive days. Subcutaneous injection of ISO(85mg/kg in saline) for two consecutive days caused a significant increase in serum cardiac marker enzymes and cardiac troponin I levels and altered serum lipid profiles. In addition significantly increased lipid peroxides and decreased activities of cellular antioxidant defense enzymeswere observed in the myocardium. However, pretreatment of ischemic rats with MP ameliorated the biochemical parameters, indicating the protective effect ofMP against ISO-induced ischemia in rats. Histopathological findings obtained for the myocardium further confirmed the biochemical findings. It is concluded that MP exhibits cardioprotective activity against ISO-induced oxidative stress through its direct cytotoxic radical-scavenging activities. It is also plausible that MP contributed to endogenous antioxidant enzyme activity via inhibition of lipid peroxidation.
AB - Propolis contains high concentrations of polyphenols, flavonoids, tannins, ascorbic acid, and reducing sugars and proteins. Malaysian Propolis (MP) has been reported to exhibit high 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical-scavenging activity and ferric reducing antioxidant power (FRAP) values. Herein, we report the antioxidant properties and cardioprotective properties of MP in isoproterenol- (ISO-) inducedmyocardial infarction in rats.MaleWistar rats ( = 32) were pretreated orallywith an ethanol extract ofMP (100mg/kg/day) for 30 consecutive days. Subcutaneous injection of ISO(85mg/kg in saline) for two consecutive days caused a significant increase in serum cardiac marker enzymes and cardiac troponin I levels and altered serum lipid profiles. In addition significantly increased lipid peroxides and decreased activities of cellular antioxidant defense enzymeswere observed in the myocardium. However, pretreatment of ischemic rats with MP ameliorated the biochemical parameters, indicating the protective effect ofMP against ISO-induced ischemia in rats. Histopathological findings obtained for the myocardium further confirmed the biochemical findings. It is concluded that MP exhibits cardioprotective activity against ISO-induced oxidative stress through its direct cytotoxic radical-scavenging activities. It is also plausible that MP contributed to endogenous antioxidant enzyme activity via inhibition of lipid peroxidation.
UR - http://www.scopus.com/inward/record.url?scp=85013436231&partnerID=8YFLogxK
U2 - 10.1155/2017/5370545
DO - 10.1155/2017/5370545
M3 - Article
C2 - 28261310
AN - SCOPUS:85013436231
SN - 1741-427X
VL - 2017
JO - Evidence-Based Complementary and Alternative Medicine
JF - Evidence-Based Complementary and Alternative Medicine
M1 - 5370545
ER -