Antioxidant properties and cardioprotective mechanism of Malaysian propolis in rats

Romana Ahmed, E. M. Tanvir, Md Sakib Hossen, Rizwana Afroz, Istiyak Ahmmed, Nur E.Noushin Rumpa, Sudip Paul, Siew Hua Gan, Siti Amrah Sulaiman, Md Ibrahim Khalil

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Abstract

Propolis contains high concentrations of polyphenols, flavonoids, tannins, ascorbic acid, and reducing sugars and proteins. Malaysian Propolis (MP) has been reported to exhibit high 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical-scavenging activity and ferric reducing antioxidant power (FRAP) values. Herein, we report the antioxidant properties and cardioprotective properties of MP in isoproterenol- (ISO-) inducedmyocardial infarction in rats.MaleWistar rats ( = 32) were pretreated orallywith an ethanol extract ofMP (100mg/kg/day) for 30 consecutive days. Subcutaneous injection of ISO(85mg/kg in saline) for two consecutive days caused a significant increase in serum cardiac marker enzymes and cardiac troponin I levels and altered serum lipid profiles. In addition significantly increased lipid peroxides and decreased activities of cellular antioxidant defense enzymeswere observed in the myocardium. However, pretreatment of ischemic rats with MP ameliorated the biochemical parameters, indicating the protective effect ofMP against ISO-induced ischemia in rats. Histopathological findings obtained for the myocardium further confirmed the biochemical findings. It is concluded that MP exhibits cardioprotective activity against ISO-induced oxidative stress through its direct cytotoxic radical-scavenging activities. It is also plausible that MP contributed to endogenous antioxidant enzyme activity via inhibition of lipid peroxidation.

Original languageEnglish
Article number5370545
Number of pages11
JournalEvidence-based Complementary and Alternative Medicine
Volume2017
DOIs
Publication statusPublished - 2 Feb 2017
Externally publishedYes

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