TY - JOUR
T1 - Antioxidant capabilities of Litsea garciae bark extracts and their relation to the phytochemical compositions
AU - Raduan, Siti Zaleha
AU - Ahmed, Qamar Uddin
AU - Kasmuri, Abdul Razak
AU - Rusmili, Muhamad Rusdi Ahmad
AU - Mia, Md Abdur Rashid
AU - Wan Sulaiman, Wan Mohd Azizi
AU - Mahmood, Muhammad Hamdi
AU - Shaikh, Mohd Farooq
N1 - Publisher Copyright:
© 2022, Malaysian Society of Applied Biology. All rights reserved.
PY - 2022/3
Y1 - 2022/3
N2 - The plant species belonging to the Litsea genus are widely investigated due to their nutritional and medicinal purposes. In this regard, this study is another similar sincere effort in which the antioxidant property and phytochemical composition of Litsea garciae (L. garciae) bark’s hexane, chloroform, methanol, and aqueous extracts were evaluated to confirm its traditional benefits. The total flavonoid content (TFC) and total phenolic content (TPC) were determined first, followed by an assessment of in vitro antioxidant activity using the DPPH and FRAP assays. The composition of the secondary metabolites was determined using Ultra-High-Performance Liquid Chromatography-Mass Spectrometry (UHPLC-MS). As a result, methanol extract was recorded to have the highest TPC value aligned with its positive appearance in phytochemical screening. Its antioxidant capacity indicated the least IC50. The results indicated that the significant free radical scavenging activity was due to the methanolic extract’s high phenolic content. The secondary metabolites found in the methanol extract varied significantly according to UHPLC-MS analysis. The major phenolic compounds were found including N-trans-feruloyl-4-O-methyldopamine, N-cis-feruloyltyramine, epicatechin-(4beta->6)-epicatechin-(2beta->7,4beta->8)-epicatechin, 7-Hydroxy-3-(4-methoxyphenyl)-4-propyl-2H-1-benzopyran-2-one and 9-O-Methylneodunol. In general, the results indicate that L. garciae bark may be a promising source of novel natural compounds with antioxidative properties.
AB - The plant species belonging to the Litsea genus are widely investigated due to their nutritional and medicinal purposes. In this regard, this study is another similar sincere effort in which the antioxidant property and phytochemical composition of Litsea garciae (L. garciae) bark’s hexane, chloroform, methanol, and aqueous extracts were evaluated to confirm its traditional benefits. The total flavonoid content (TFC) and total phenolic content (TPC) were determined first, followed by an assessment of in vitro antioxidant activity using the DPPH and FRAP assays. The composition of the secondary metabolites was determined using Ultra-High-Performance Liquid Chromatography-Mass Spectrometry (UHPLC-MS). As a result, methanol extract was recorded to have the highest TPC value aligned with its positive appearance in phytochemical screening. Its antioxidant capacity indicated the least IC50. The results indicated that the significant free radical scavenging activity was due to the methanolic extract’s high phenolic content. The secondary metabolites found in the methanol extract varied significantly according to UHPLC-MS analysis. The major phenolic compounds were found including N-trans-feruloyl-4-O-methyldopamine, N-cis-feruloyltyramine, epicatechin-(4beta->6)-epicatechin-(2beta->7,4beta->8)-epicatechin, 7-Hydroxy-3-(4-methoxyphenyl)-4-propyl-2H-1-benzopyran-2-one and 9-O-Methylneodunol. In general, the results indicate that L. garciae bark may be a promising source of novel natural compounds with antioxidative properties.
KW - Antioxidant
KW - aqueous
KW - chloroform
KW - hexane
KW - Litsea garciae
KW - methanol
UR - https://www.scopus.com/pages/publications/85129668736
U2 - 10.55230/MABJOURNAL.V51I1.2038
DO - 10.55230/MABJOURNAL.V51I1.2038
M3 - Article
AN - SCOPUS:85129668736
SN - 0126-8643
VL - 51
SP - 99
EP - 118
JO - Malaysian Applied Biology
JF - Malaysian Applied Biology
IS - 1
ER -