TY - JOUR
T1 - Antioxidant activity of the phenolic leaf extracts from monechma ciliatum in stabilization of corn oil
AU - Mariod, Abdalbasit Adam
AU - Ibrahim, Ramlah Mohamad
AU - Ismail, Maznah
AU - Ismail, Norsharina
N1 - Funding Information:
chromatogram of phenolic compounds in black mahlab leaves. Detection was at 280 nm. a CME. Peak: 6 p-coumaric 7 vanillin. b EAE. Peak: 6 p-coumaric 7 vanillin. c HE. Peak: 6 p-coumaric 7 vanillin, 8 ferulic acid. d Standards of phenolic acids. Peak: 1 gallic acid, 2 (?)- catechin, 3 chlorogenic acid, 4 hydroxybenzoic acid, 5 syringic acid, 6 p-coumaric 7 vanillin, 8 ferulic acid, 9 quercetin Acknowledgments The first author is grateful to IBS/UPM for the senior research fellowship granted during the period of this work. We thank Siti Muskinah, Laboratory of Molecular Biomedicine IBS/UPM for analysis in HPLC-DAD equipment and excellent technical expertise needed for this work. This study was funded by Intensifying Research Priority Areas (IRPA) grants provided by Government of Malaysia and Universiti Putra Malaysia.
PY - 2010/1
Y1 - 2010/1
N2 - The total phenolic content and the antioxidant potential of methanolic extract (ME), ethyl acetate extract (EAE), and hexane extract (HE) from Monechma ciliatum leaves (MCL) were evaluated. The Folin-Ciocalteu, β-carotene bleaching, the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging and the accelerated oxidation methods were used for evaluation. Both the extraction yield and the antioxidant activity (AOA) were strongly dependent on the solvent. Among the extracts, ME exhibited highest total phenolic compounds (TPC) and IC50 values for DPPH, followed by EAE and HE, respectively. Peroxide value (PV), anisidine value (AV) conjugated dienes (CD), and thiobarbituric acid reactive substances (TBARS) were taken as the parameters for evaluation of stabilization efficacy of MCL extracts and results revealed MCL to be a potent antioxidant for the stabilization of corn oil. As a general trend, increased AOA was observed for increased extract concentration. The predominant phenolic compounds identified by HPLC-DAD in MCL extracts were p-coumaric acid, vanillin and ferulic acid.
AB - The total phenolic content and the antioxidant potential of methanolic extract (ME), ethyl acetate extract (EAE), and hexane extract (HE) from Monechma ciliatum leaves (MCL) were evaluated. The Folin-Ciocalteu, β-carotene bleaching, the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging and the accelerated oxidation methods were used for evaluation. Both the extraction yield and the antioxidant activity (AOA) were strongly dependent on the solvent. Among the extracts, ME exhibited highest total phenolic compounds (TPC) and IC50 values for DPPH, followed by EAE and HE, respectively. Peroxide value (PV), anisidine value (AV) conjugated dienes (CD), and thiobarbituric acid reactive substances (TBARS) were taken as the parameters for evaluation of stabilization efficacy of MCL extracts and results revealed MCL to be a potent antioxidant for the stabilization of corn oil. As a general trend, increased AOA was observed for increased extract concentration. The predominant phenolic compounds identified by HPLC-DAD in MCL extracts were p-coumaric acid, vanillin and ferulic acid.
KW - β-Carotene bleaching assay
KW - 2,2-Diphenyl-1- picrylhydrazyl (DPPH)
KW - Antioxidant activity
KW - Monechma ciliatum
KW - Oil oxidation
KW - Total phenolic content
UR - http://www.scopus.com/inward/record.url?scp=73849138875&partnerID=8YFLogxK
U2 - 10.1007/s11746-009-1474-4
DO - 10.1007/s11746-009-1474-4
M3 - Article
AN - SCOPUS:73849138875
SN - 0003-021X
VL - 87
SP - 35
EP - 43
JO - Journal of the American Oil Chemists' Society
JF - Journal of the American Oil Chemists' Society
IS - 1
ER -