Antimicrobial and biosensing ultrasound-responsive lysozyme-shelled microbubbles

Francesca Cavalieri, Laura Micheli, Subramanian Kaliappan, Boon M. Teo, Meifang Zhou, Giuseppe Palleschi, Muthupandian Ashokkumar

Research output: Contribution to journalArticleResearchpeer-review

27 Citations (Scopus)


Air-filled lysozyme microbubbles (LSMBs) were engineered as a support for the immobilization of gold nanoparticles and an enzyme, alkaline phosphatase, in order to develop micro-antimicrobial and biosensing devices. Gold nanoparticles immobilized on LSMBs significantly improved the antimicrobial efficacy of the microbubbles against M. lysodeikticus. The surface functionalization of the microbubbles with gold nanoparticles did not affect their echogenicity when exposed to an ultrasound imaging probe. Alkaline phosphatase was conjugated on the surface of microbubbles without compromising its enzymatic activity. The functionalized microbubbles were used for the detection of paraoxon in aqueous solutions.

Original languageEnglish
Pages (from-to)464-471
Number of pages8
JournalACS Applied Materials & Interfaces
Issue number2
Publication statusPublished - 23 Jan 2013
Externally publishedYes


  • alkaline phosphatase
  • antimicrobial microparticles
  • biosensing microparticles
  • gold nanoparticles
  • lysozyme
  • microbubbles

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