Antigen-specific CD8+ T cell subset distribution in lymph nodes draining the site of herpes simplex virus infection

Stephen C. Cose, Claerwen M. Jones, Morgan E. Wallace, William R. Heath, Francis R. Carbone

Research output: Contribution to journalArticleResearchpeer-review

38 Citations (Scopus)

Abstract

Inoculation with replicating virus leads to an increase in T cell numbers within lymph nodes that drain the site of infection. This increase has been associated with a nonspecific proliferation of bystander cells, with only a minority thought to be directed to the infectious agent. Such an assumption is largely based on precursor cytotoxic T lymphocyte (CTL) estimations using limiting dilution analysis. Recently, studies using more advanced molecular approaches have suggested that such functionally derived precursor frequencies considerably underestimate the proportion of T cells specific for the antigen under investigation. We have defined T cell receptor sequences characteristic of CTL populations directed to a dominant determinant of the herpes simplex virus (HSV) glycoprotein B (gB). In this investigation, we used this receptor signature as a probe to directly monitor changes occurring within lymph nodes draining the sites of active infection with HSV. We found that although lymph node CD8+ T cell numbers increase as a consequence of HSV infection, the majority of these cells are small resting cells that are not enriched for gB-specific receptors. In contrast, a significant proportion of activated T cells are highly enriched for CTL bearing gB-specific receptors. Our results are therefore consistent with a nonspecific migration of CTL precursors into the lymph nodes draining the site of infection, followed by the activation and proliferation of the antigen-specific subset that normally makes up a small proportion of the naive T cell repertoire.

Original languageEnglish
Pages (from-to)2310-2316
Number of pages7
JournalEuropean Journal of Immunology
Volume27
Issue number9
DOIs
Publication statusPublished - 1 Sep 1997

Keywords

  • Cytotoxic T lymphocyte
  • Herpes simplex virus
  • Lymph node
  • T cell receptor

Cite this