Antigen ligation triggers a conformational change within the constant domain of the alphabeta T cell receptor

Travis Clarke Beddoe, Zhenjun Chen, Craig Steven Clements, Lauren Kate Ely, Simon Robert Bushell, Julian P Vivian, Lars Kjer-Nielsen, Siew Siew Pang, Michelle Anne Dunstone, Yu Chih Liu, Whitney Alison Macdonald, Matthew Anthony Perugini, Matthew Charles James Wilce, Scott R Burrows, Anthony Wayne Purcell, Tony Tiganis, Stephen Paul Bottomley, James McCluskey, Jamie Rossjohn

Research output: Contribution to journalArticleResearchpeer-review

77 Citations (Scopus)

Abstract

Ligation of the alphabeta T cell receptor (TCR) by a specific peptide-loaded major histocompatibility complex (pMHC) molecule initiates T cell signaling via the CD3 complex. However, the initial events that link antigen recognition to T cell signal transduction remain unclear. Here we show, via fluorescence-based experiments and structural analyses, that MHC-restricted antigen recognition by the alphabeta TCR results in a specific conformational change confined to the A-B loop within the alpha chain of the constant domain (Calpha). The apparent affinity constant of this A-B loop movement mirrored that of alphabeta TCR-pMHC ligation and was observed in two alphabeta TCRs with distinct pMHC specificities. The Ag-induced A-B loop conformational change could be inhibited by fixing the juxtapositioning of the constant domains and was shown to be reversible upon pMHC disassociation. Notably, the loop movement within the Calpha domain, although specific for an agonist pMHC ligand, was not observed with a pMHC antagonist. Moreover, mutagenesis of residues within the A-B loop impaired T cell signaling in an in vitro system of antigen-specific TCR stimulation. Collectively, our findings provide a basis for the earliest molecular events that underlie Ag-induced T cell triggering.
Original languageEnglish
Pages (from-to)777 - 788
Number of pages12
JournalImmunity
Volume30
Issue number6
DOIs
Publication statusPublished - 2009

Cite this

Beddoe, Travis Clarke ; Chen, Zhenjun ; Clements, Craig Steven ; Ely, Lauren Kate ; Bushell, Simon Robert ; Vivian, Julian P ; Kjer-Nielsen, Lars ; Pang, Siew Siew ; Dunstone, Michelle Anne ; Liu, Yu Chih ; Macdonald, Whitney Alison ; Perugini, Matthew Anthony ; Wilce, Matthew Charles James ; Burrows, Scott R ; Purcell, Anthony Wayne ; Tiganis, Tony ; Bottomley, Stephen Paul ; McCluskey, James ; Rossjohn, Jamie. / Antigen ligation triggers a conformational change within the constant domain of the alphabeta T cell receptor. In: Immunity. 2009 ; Vol. 30, No. 6. pp. 777 - 788.
@article{7fb67def167e492c845aea635e7dc598,
title = "Antigen ligation triggers a conformational change within the constant domain of the alphabeta T cell receptor",
abstract = "Ligation of the alphabeta T cell receptor (TCR) by a specific peptide-loaded major histocompatibility complex (pMHC) molecule initiates T cell signaling via the CD3 complex. However, the initial events that link antigen recognition to T cell signal transduction remain unclear. Here we show, via fluorescence-based experiments and structural analyses, that MHC-restricted antigen recognition by the alphabeta TCR results in a specific conformational change confined to the A-B loop within the alpha chain of the constant domain (Calpha). The apparent affinity constant of this A-B loop movement mirrored that of alphabeta TCR-pMHC ligation and was observed in two alphabeta TCRs with distinct pMHC specificities. The Ag-induced A-B loop conformational change could be inhibited by fixing the juxtapositioning of the constant domains and was shown to be reversible upon pMHC disassociation. Notably, the loop movement within the Calpha domain, although specific for an agonist pMHC ligand, was not observed with a pMHC antagonist. Moreover, mutagenesis of residues within the A-B loop impaired T cell signaling in an in vitro system of antigen-specific TCR stimulation. Collectively, our findings provide a basis for the earliest molecular events that underlie Ag-induced T cell triggering.",
author = "Beddoe, {Travis Clarke} and Zhenjun Chen and Clements, {Craig Steven} and Ely, {Lauren Kate} and Bushell, {Simon Robert} and Vivian, {Julian P} and Lars Kjer-Nielsen and Pang, {Siew Siew} and Dunstone, {Michelle Anne} and Liu, {Yu Chih} and Macdonald, {Whitney Alison} and Perugini, {Matthew Anthony} and Wilce, {Matthew Charles James} and Burrows, {Scott R} and Purcell, {Anthony Wayne} and Tony Tiganis and Bottomley, {Stephen Paul} and James McCluskey and Jamie Rossjohn",
year = "2009",
doi = "10.1016/j.immuni.2009.03.018",
language = "English",
volume = "30",
pages = "777 -- 788",
journal = "Immunity",
issn = "1074-7613",
publisher = "Elsevier",
number = "6",

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Beddoe, TC, Chen, Z, Clements, CS, Ely, LK, Bushell, SR, Vivian, JP, Kjer-Nielsen, L, Pang, SS, Dunstone, MA, Liu, YC, Macdonald, WA, Perugini, MA, Wilce, MCJ, Burrows, SR, Purcell, AW, Tiganis, T, Bottomley, SP, McCluskey, J & Rossjohn, J 2009, 'Antigen ligation triggers a conformational change within the constant domain of the alphabeta T cell receptor', Immunity, vol. 30, no. 6, pp. 777 - 788. https://doi.org/10.1016/j.immuni.2009.03.018

Antigen ligation triggers a conformational change within the constant domain of the alphabeta T cell receptor. / Beddoe, Travis Clarke; Chen, Zhenjun; Clements, Craig Steven; Ely, Lauren Kate; Bushell, Simon Robert; Vivian, Julian P; Kjer-Nielsen, Lars; Pang, Siew Siew; Dunstone, Michelle Anne; Liu, Yu Chih; Macdonald, Whitney Alison; Perugini, Matthew Anthony; Wilce, Matthew Charles James; Burrows, Scott R; Purcell, Anthony Wayne; Tiganis, Tony; Bottomley, Stephen Paul; McCluskey, James; Rossjohn, Jamie.

In: Immunity, Vol. 30, No. 6, 2009, p. 777 - 788.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Antigen ligation triggers a conformational change within the constant domain of the alphabeta T cell receptor

AU - Beddoe, Travis Clarke

AU - Chen, Zhenjun

AU - Clements, Craig Steven

AU - Ely, Lauren Kate

AU - Bushell, Simon Robert

AU - Vivian, Julian P

AU - Kjer-Nielsen, Lars

AU - Pang, Siew Siew

AU - Dunstone, Michelle Anne

AU - Liu, Yu Chih

AU - Macdonald, Whitney Alison

AU - Perugini, Matthew Anthony

AU - Wilce, Matthew Charles James

AU - Burrows, Scott R

AU - Purcell, Anthony Wayne

AU - Tiganis, Tony

AU - Bottomley, Stephen Paul

AU - McCluskey, James

AU - Rossjohn, Jamie

PY - 2009

Y1 - 2009

N2 - Ligation of the alphabeta T cell receptor (TCR) by a specific peptide-loaded major histocompatibility complex (pMHC) molecule initiates T cell signaling via the CD3 complex. However, the initial events that link antigen recognition to T cell signal transduction remain unclear. Here we show, via fluorescence-based experiments and structural analyses, that MHC-restricted antigen recognition by the alphabeta TCR results in a specific conformational change confined to the A-B loop within the alpha chain of the constant domain (Calpha). The apparent affinity constant of this A-B loop movement mirrored that of alphabeta TCR-pMHC ligation and was observed in two alphabeta TCRs with distinct pMHC specificities. The Ag-induced A-B loop conformational change could be inhibited by fixing the juxtapositioning of the constant domains and was shown to be reversible upon pMHC disassociation. Notably, the loop movement within the Calpha domain, although specific for an agonist pMHC ligand, was not observed with a pMHC antagonist. Moreover, mutagenesis of residues within the A-B loop impaired T cell signaling in an in vitro system of antigen-specific TCR stimulation. Collectively, our findings provide a basis for the earliest molecular events that underlie Ag-induced T cell triggering.

AB - Ligation of the alphabeta T cell receptor (TCR) by a specific peptide-loaded major histocompatibility complex (pMHC) molecule initiates T cell signaling via the CD3 complex. However, the initial events that link antigen recognition to T cell signal transduction remain unclear. Here we show, via fluorescence-based experiments and structural analyses, that MHC-restricted antigen recognition by the alphabeta TCR results in a specific conformational change confined to the A-B loop within the alpha chain of the constant domain (Calpha). The apparent affinity constant of this A-B loop movement mirrored that of alphabeta TCR-pMHC ligation and was observed in two alphabeta TCRs with distinct pMHC specificities. The Ag-induced A-B loop conformational change could be inhibited by fixing the juxtapositioning of the constant domains and was shown to be reversible upon pMHC disassociation. Notably, the loop movement within the Calpha domain, although specific for an agonist pMHC ligand, was not observed with a pMHC antagonist. Moreover, mutagenesis of residues within the A-B loop impaired T cell signaling in an in vitro system of antigen-specific TCR stimulation. Collectively, our findings provide a basis for the earliest molecular events that underlie Ag-induced T cell triggering.

UR - http://www.sciencedirect.com/science/article/pii/S1074761309001952

U2 - 10.1016/j.immuni.2009.03.018

DO - 10.1016/j.immuni.2009.03.018

M3 - Article

VL - 30

SP - 777

EP - 788

JO - Immunity

JF - Immunity

SN - 1074-7613

IS - 6

ER -