Antigen-induced neutrophil chemotactic factor from cloned human T lymphocytes

P. Maestrelli, R. E. O'Hehir, J. R. Lamb, J. J. Tsai, O. Cromwell, A. B. Kay

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Abstract

Here we report the presence of a low molecular weight 10,000 neutrophil chemotactic factor (NCF) in the supernatants of activated human T lymphocytes. Appreciable amounts of the 10,000 MW NCF were generated by peripheral blood mononuclear cells (PBMC) stimulated with anti-CD3 antibody, and comparable NCF was secreted by both long-term human T-helper (CD4+) cell lines reactive with house dust mite (Dermatophagoides farinae) and influenza A virus-immune T-cell clones. When the cloned T cells were stimulated with specific antigen in the presence of irradiated accessory cells (AC) or insolubilized anti-CD3 antibody the 10,000 MW NCF was readily identifiable in 24-hr culture supernatants. Cultures of AC and antigen alone produced negligible neutrophil chemotactic activity, as did control cultures using an irrelavant allergen (mixed grass pollen). These findings indicate that the 10,000 MW NCF may be T-lymphocyte derived and that formation and release are dependent upon stimulation via the antigen receptor.

Original languageEnglish
Pages (from-to)605-609
Number of pages5
JournalImmunology
Volume65
Issue number4
Publication statusPublished - 1 Dec 1988
Externally publishedYes

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