Antidepressants in Surface Waters: Fluoxetine Influences Mosquitofish Anxiety-Related Behavior at Environmentally Relevant Levels

Jake M. Martin, Michael G. Bertram, Minna Saaristo, Jack B. Fursdon, Stephanie L. Hannington, Bryan W. Brooks, S. Rebekah Burket, Rachel A. Mole, Nicholas D.S. Deal, Bob B.M. Wong

Research output: Contribution to journalArticleResearchpeer-review

3 Citations (Scopus)

Abstract

Pharmaceutical contamination is an increasing problem globally. In this regard, the selective serotonin reuptake inhibitors (SSRIs) - a group of antidepressants - are particularly concerning. By disrupting the serotonergic system, SSRIs have the potential to affect ecologically important behaviors in exposed wildlife. Despite this, the nature and magnitude of behavioral perturbations resulting from environmentally relevant SSRI exposure among species is poorly understood. Accordingly, we investigated the effects of two field-realistic levels of the SSRI fluoxetine (61 and 352 ng/L) on sociability and anxiety-related behaviors in eastern mosquitofish (Gambusia holbrooki) for 28 days. Additionally, we measured whole-body tissue concentrations of fluoxetine and norfluoxetine. We found that fluoxetine altered anxiety-related behavior but not sociability. Specifically, female fish showed reduced anxiety-related behavior at the lower treatment level, while males showed an increase at the higher treatment level. In addition, we report a biomass-dependent and sex-specific accumulation of fluoxetine and norfluoxetine, with smaller fish showing higher relative tissue concentrations, with this relationship being more pronounced in males. Our study provides evidence for nonmonotonic and sex-specific effects of fluoxetine exposure at field-realistic concentrations. More broadly, our study demonstrated that neuroactive pharmaceuticals, such as fluoxetine, can affect aquatic life by causing subtle but important shifts in ecologically relevant behaviors.

Original languageEnglish
Pages (from-to)6035-6043
Number of pages9
JournalEnvironmental Science and Technology
Volume53
Issue number10
DOIs
Publication statusPublished - 21 May 2019

Cite this

Martin, Jake M. ; Bertram, Michael G. ; Saaristo, Minna ; Fursdon, Jack B. ; Hannington, Stephanie L. ; Brooks, Bryan W. ; Burket, S. Rebekah ; Mole, Rachel A. ; Deal, Nicholas D.S. ; Wong, Bob B.M. / Antidepressants in Surface Waters : Fluoxetine Influences Mosquitofish Anxiety-Related Behavior at Environmentally Relevant Levels. In: Environmental Science and Technology. 2019 ; Vol. 53, No. 10. pp. 6035-6043.
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abstract = "Pharmaceutical contamination is an increasing problem globally. In this regard, the selective serotonin reuptake inhibitors (SSRIs) - a group of antidepressants - are particularly concerning. By disrupting the serotonergic system, SSRIs have the potential to affect ecologically important behaviors in exposed wildlife. Despite this, the nature and magnitude of behavioral perturbations resulting from environmentally relevant SSRI exposure among species is poorly understood. Accordingly, we investigated the effects of two field-realistic levels of the SSRI fluoxetine (61 and 352 ng/L) on sociability and anxiety-related behaviors in eastern mosquitofish (Gambusia holbrooki) for 28 days. Additionally, we measured whole-body tissue concentrations of fluoxetine and norfluoxetine. We found that fluoxetine altered anxiety-related behavior but not sociability. Specifically, female fish showed reduced anxiety-related behavior at the lower treatment level, while males showed an increase at the higher treatment level. In addition, we report a biomass-dependent and sex-specific accumulation of fluoxetine and norfluoxetine, with smaller fish showing higher relative tissue concentrations, with this relationship being more pronounced in males. Our study provides evidence for nonmonotonic and sex-specific effects of fluoxetine exposure at field-realistic concentrations. More broadly, our study demonstrated that neuroactive pharmaceuticals, such as fluoxetine, can affect aquatic life by causing subtle but important shifts in ecologically relevant behaviors.",
author = "Martin, {Jake M.} and Bertram, {Michael G.} and Minna Saaristo and Fursdon, {Jack B.} and Hannington, {Stephanie L.} and Brooks, {Bryan W.} and Burket, {S. Rebekah} and Mole, {Rachel A.} and Deal, {Nicholas D.S.} and Wong, {Bob B.M.}",
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Antidepressants in Surface Waters : Fluoxetine Influences Mosquitofish Anxiety-Related Behavior at Environmentally Relevant Levels. / Martin, Jake M.; Bertram, Michael G.; Saaristo, Minna; Fursdon, Jack B.; Hannington, Stephanie L.; Brooks, Bryan W.; Burket, S. Rebekah; Mole, Rachel A.; Deal, Nicholas D.S.; Wong, Bob B.M.

In: Environmental Science and Technology, Vol. 53, No. 10, 21.05.2019, p. 6035-6043.

Research output: Contribution to journalArticleResearchpeer-review

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T1 - Antidepressants in Surface Waters

T2 - Fluoxetine Influences Mosquitofish Anxiety-Related Behavior at Environmentally Relevant Levels

AU - Martin, Jake M.

AU - Bertram, Michael G.

AU - Saaristo, Minna

AU - Fursdon, Jack B.

AU - Hannington, Stephanie L.

AU - Brooks, Bryan W.

AU - Burket, S. Rebekah

AU - Mole, Rachel A.

AU - Deal, Nicholas D.S.

AU - Wong, Bob B.M.

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