Antibodies to a single, conserved epitope in Anopheles APN1 inhibit universal transmission of Plasmodium falciparum and Plasmodium vivax malaria

Jennifer S Armistead, Isabelle Morlais, Derrick K Mathias, Juliette G Jardim, Jaimy Joy, Arthur Fridman, Adam C Finnefrock, Ansu Bagchi, Magdalena Plebanski, Diana G Scorpio, Thomas S Churcher, Natalie Borg, Jetsumon Sattabongkot, Rhoel R Dinglasan

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Abstract

Malaria transmission-blocking vaccines (TBVs) represent a promising approach for the elimination and eradication of this disease. AnAPN1 is a lead TBV candidate that targets a surface antigen on the midgut of the obligate vector of the Plasmodium parasite, the Anopheles mosquito. In this study, we demonstrated that antibodies targeting AnAPN1 block transmission of Plasmodium falciparum and Plasmodium vivax across distantly related anopheline species in countries to which malaria is endemic. Using a biochemical and immunological approach, we determined that the mechanism of action for this phenomenon stems from antibody recognition of a single protective epitope on AnAPN1, which we found to be immunogenic in murine and nonhuman primate models and highly conserved among anophelines. These data indicate that AnAPN1 meets the established target product profile for TBVs and suggest a potential key role for an AnAPN1-based panmalaria TBV in the effort to eradicate malaria.
Original languageEnglish
Pages (from-to)818 - 829
Number of pages12
JournalInfection and Immunity
Volume82
Issue number2
DOIs
Publication statusPublished - 2014

Cite this

Armistead, J. S., Morlais, I., Mathias, D. K., Jardim, J. G., Joy, J., Fridman, A., ... Dinglasan, R. R. (2014). Antibodies to a single, conserved epitope in Anopheles APN1 inhibit universal transmission of Plasmodium falciparum and Plasmodium vivax malaria. Infection and Immunity, 82(2), 818 - 829. https://doi.org/10.1128/IAI.01222-13
Armistead, Jennifer S ; Morlais, Isabelle ; Mathias, Derrick K ; Jardim, Juliette G ; Joy, Jaimy ; Fridman, Arthur ; Finnefrock, Adam C ; Bagchi, Ansu ; Plebanski, Magdalena ; Scorpio, Diana G ; Churcher, Thomas S ; Borg, Natalie ; Sattabongkot, Jetsumon ; Dinglasan, Rhoel R. / Antibodies to a single, conserved epitope in Anopheles APN1 inhibit universal transmission of Plasmodium falciparum and Plasmodium vivax malaria. In: Infection and Immunity. 2014 ; Vol. 82, No. 2. pp. 818 - 829.
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title = "Antibodies to a single, conserved epitope in Anopheles APN1 inhibit universal transmission of Plasmodium falciparum and Plasmodium vivax malaria",
abstract = "Malaria transmission-blocking vaccines (TBVs) represent a promising approach for the elimination and eradication of this disease. AnAPN1 is a lead TBV candidate that targets a surface antigen on the midgut of the obligate vector of the Plasmodium parasite, the Anopheles mosquito. In this study, we demonstrated that antibodies targeting AnAPN1 block transmission of Plasmodium falciparum and Plasmodium vivax across distantly related anopheline species in countries to which malaria is endemic. Using a biochemical and immunological approach, we determined that the mechanism of action for this phenomenon stems from antibody recognition of a single protective epitope on AnAPN1, which we found to be immunogenic in murine and nonhuman primate models and highly conserved among anophelines. These data indicate that AnAPN1 meets the established target product profile for TBVs and suggest a potential key role for an AnAPN1-based panmalaria TBV in the effort to eradicate malaria.",
author = "Armistead, {Jennifer S} and Isabelle Morlais and Mathias, {Derrick K} and Jardim, {Juliette G} and Jaimy Joy and Arthur Fridman and Finnefrock, {Adam C} and Ansu Bagchi and Magdalena Plebanski and Scorpio, {Diana G} and Churcher, {Thomas S} and Natalie Borg and Jetsumon Sattabongkot and Dinglasan, {Rhoel R}",
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Armistead, JS, Morlais, I, Mathias, DK, Jardim, JG, Joy, J, Fridman, A, Finnefrock, AC, Bagchi, A, Plebanski, M, Scorpio, DG, Churcher, TS, Borg, N, Sattabongkot, J & Dinglasan, RR 2014, 'Antibodies to a single, conserved epitope in Anopheles APN1 inhibit universal transmission of Plasmodium falciparum and Plasmodium vivax malaria', Infection and Immunity, vol. 82, no. 2, pp. 818 - 829. https://doi.org/10.1128/IAI.01222-13

Antibodies to a single, conserved epitope in Anopheles APN1 inhibit universal transmission of Plasmodium falciparum and Plasmodium vivax malaria. / Armistead, Jennifer S; Morlais, Isabelle; Mathias, Derrick K; Jardim, Juliette G; Joy, Jaimy; Fridman, Arthur; Finnefrock, Adam C; Bagchi, Ansu; Plebanski, Magdalena; Scorpio, Diana G; Churcher, Thomas S; Borg, Natalie; Sattabongkot, Jetsumon; Dinglasan, Rhoel R.

In: Infection and Immunity, Vol. 82, No. 2, 2014, p. 818 - 829.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Antibodies to a single, conserved epitope in Anopheles APN1 inhibit universal transmission of Plasmodium falciparum and Plasmodium vivax malaria

AU - Armistead, Jennifer S

AU - Morlais, Isabelle

AU - Mathias, Derrick K

AU - Jardim, Juliette G

AU - Joy, Jaimy

AU - Fridman, Arthur

AU - Finnefrock, Adam C

AU - Bagchi, Ansu

AU - Plebanski, Magdalena

AU - Scorpio, Diana G

AU - Churcher, Thomas S

AU - Borg, Natalie

AU - Sattabongkot, Jetsumon

AU - Dinglasan, Rhoel R

PY - 2014

Y1 - 2014

N2 - Malaria transmission-blocking vaccines (TBVs) represent a promising approach for the elimination and eradication of this disease. AnAPN1 is a lead TBV candidate that targets a surface antigen on the midgut of the obligate vector of the Plasmodium parasite, the Anopheles mosquito. In this study, we demonstrated that antibodies targeting AnAPN1 block transmission of Plasmodium falciparum and Plasmodium vivax across distantly related anopheline species in countries to which malaria is endemic. Using a biochemical and immunological approach, we determined that the mechanism of action for this phenomenon stems from antibody recognition of a single protective epitope on AnAPN1, which we found to be immunogenic in murine and nonhuman primate models and highly conserved among anophelines. These data indicate that AnAPN1 meets the established target product profile for TBVs and suggest a potential key role for an AnAPN1-based panmalaria TBV in the effort to eradicate malaria.

AB - Malaria transmission-blocking vaccines (TBVs) represent a promising approach for the elimination and eradication of this disease. AnAPN1 is a lead TBV candidate that targets a surface antigen on the midgut of the obligate vector of the Plasmodium parasite, the Anopheles mosquito. In this study, we demonstrated that antibodies targeting AnAPN1 block transmission of Plasmodium falciparum and Plasmodium vivax across distantly related anopheline species in countries to which malaria is endemic. Using a biochemical and immunological approach, we determined that the mechanism of action for this phenomenon stems from antibody recognition of a single protective epitope on AnAPN1, which we found to be immunogenic in murine and nonhuman primate models and highly conserved among anophelines. These data indicate that AnAPN1 meets the established target product profile for TBVs and suggest a potential key role for an AnAPN1-based panmalaria TBV in the effort to eradicate malaria.

UR - http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3911399/pdf/zii818.pdf

U2 - 10.1128/IAI.01222-13

DO - 10.1128/IAI.01222-13

M3 - Article

VL - 82

SP - 818

EP - 829

JO - Infection and Immunity

JF - Infection and Immunity

SN - 0019-9567

IS - 2

ER -