Antibodies that block or activate mouse b cell activating factor of the tumor necrosis factor (TNF) family (baff), respectively, induce b cell depletion or b cell hyperplasia

Christine Kowalczyk-Quintas, Sonia Schuepbach-Mallepell, Michele Vigolo, Laure Willen, Aubry Tardivel, Cristian R. Smulski, Timothy S. Zheng, Jennifer Gommerman, Henry Hess, Jacques Eric Gottenberg, Fabienne Mackay, Olivier Donze, Pascal Schneider

Research output: Contribution to journalArticleResearchpeer-review

6 Citations (Scopus)

Abstract

B cell activating factor of the TNF family (BAFF), also known as B lymphocyte stimulator, is a ligand required for the generation and maintenance of B lymphocytes. In this study, the ability of different monoclonal antibodies to recognize, inhibit, or activate mouse BAFF was investigated. One of them, a mouse IgG1 named Sandy-2, prevented the binding of BAFF to all of its receptors, BAFF receptor, transmembrane activator and calcium modulating ligand interactor, and B cell maturation antigen, at a stoichiometric ratio; blocked the activity of mouse BAFF on a variety of cell-based reporter assays; and antagonized the prosurvival action of BAFF on primary mouse B cells in vitro. A single administration of Sandy-2 in mice induced B cell depletion within 2 weeks, down to levels close to those observed in BAFF-deficient mice. This depletion could then be maintained with a chronic treatment. Sandy-2 and a previously described rat IgG1 antibody, 5A8, also formed a pair suitable for the sensitive detection of endogenous circulating BAFF by ELISA or using a homogenous assay. Interestingly, 5A8 and Sandy-5 displayed activities opposite to that of Sandy-2 by stimulating recombinant BAFF in vitro and endogenous BAFF in vivo. These tools will prove useful for the detection and functional manipulation of endogenous mouse BAFF and provide an alternative to the widely used BAFF receptor-Fc decoy receptor for the specific depletion of BAFF in mice.

Original languageEnglish
Pages (from-to)19826-19834
Number of pages9
JournalJournal of Biological Chemistry
Volume291
Issue number38
DOIs
Publication statusPublished - 16 Sep 2016

Cite this

Kowalczyk-Quintas, Christine ; Schuepbach-Mallepell, Sonia ; Vigolo, Michele ; Willen, Laure ; Tardivel, Aubry ; Smulski, Cristian R. ; Zheng, Timothy S. ; Gommerman, Jennifer ; Hess, Henry ; Gottenberg, Jacques Eric ; Mackay, Fabienne ; Donze, Olivier ; Schneider, Pascal. / Antibodies that block or activate mouse b cell activating factor of the tumor necrosis factor (TNF) family (baff), respectively, induce b cell depletion or b cell hyperplasia. In: Journal of Biological Chemistry. 2016 ; Vol. 291, No. 38. pp. 19826-19834.
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title = "Antibodies that block or activate mouse b cell activating factor of the tumor necrosis factor (TNF) family (baff), respectively, induce b cell depletion or b cell hyperplasia",
abstract = "B cell activating factor of the TNF family (BAFF), also known as B lymphocyte stimulator, is a ligand required for the generation and maintenance of B lymphocytes. In this study, the ability of different monoclonal antibodies to recognize, inhibit, or activate mouse BAFF was investigated. One of them, a mouse IgG1 named Sandy-2, prevented the binding of BAFF to all of its receptors, BAFF receptor, transmembrane activator and calcium modulating ligand interactor, and B cell maturation antigen, at a stoichiometric ratio; blocked the activity of mouse BAFF on a variety of cell-based reporter assays; and antagonized the prosurvival action of BAFF on primary mouse B cells in vitro. A single administration of Sandy-2 in mice induced B cell depletion within 2 weeks, down to levels close to those observed in BAFF-deficient mice. This depletion could then be maintained with a chronic treatment. Sandy-2 and a previously described rat IgG1 antibody, 5A8, also formed a pair suitable for the sensitive detection of endogenous circulating BAFF by ELISA or using a homogenous assay. Interestingly, 5A8 and Sandy-5 displayed activities opposite to that of Sandy-2 by stimulating recombinant BAFF in vitro and endogenous BAFF in vivo. These tools will prove useful for the detection and functional manipulation of endogenous mouse BAFF and provide an alternative to the widely used BAFF receptor-Fc decoy receptor for the specific depletion of BAFF in mice.",
author = "Christine Kowalczyk-Quintas and Sonia Schuepbach-Mallepell and Michele Vigolo and Laure Willen and Aubry Tardivel and Smulski, {Cristian R.} and Zheng, {Timothy S.} and Jennifer Gommerman and Henry Hess and Gottenberg, {Jacques Eric} and Fabienne Mackay and Olivier Donze and Pascal Schneider",
year = "2016",
month = "9",
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doi = "10.1074/jbc.M116.725929",
language = "English",
volume = "291",
pages = "19826--19834",
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Kowalczyk-Quintas, C, Schuepbach-Mallepell, S, Vigolo, M, Willen, L, Tardivel, A, Smulski, CR, Zheng, TS, Gommerman, J, Hess, H, Gottenberg, JE, Mackay, F, Donze, O & Schneider, P 2016, 'Antibodies that block or activate mouse b cell activating factor of the tumor necrosis factor (TNF) family (baff), respectively, induce b cell depletion or b cell hyperplasia', Journal of Biological Chemistry, vol. 291, no. 38, pp. 19826-19834. https://doi.org/10.1074/jbc.M116.725929

Antibodies that block or activate mouse b cell activating factor of the tumor necrosis factor (TNF) family (baff), respectively, induce b cell depletion or b cell hyperplasia. / Kowalczyk-Quintas, Christine; Schuepbach-Mallepell, Sonia; Vigolo, Michele; Willen, Laure; Tardivel, Aubry; Smulski, Cristian R.; Zheng, Timothy S.; Gommerman, Jennifer; Hess, Henry; Gottenberg, Jacques Eric; Mackay, Fabienne; Donze, Olivier; Schneider, Pascal.

In: Journal of Biological Chemistry, Vol. 291, No. 38, 16.09.2016, p. 19826-19834.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Antibodies that block or activate mouse b cell activating factor of the tumor necrosis factor (TNF) family (baff), respectively, induce b cell depletion or b cell hyperplasia

AU - Kowalczyk-Quintas, Christine

AU - Schuepbach-Mallepell, Sonia

AU - Vigolo, Michele

AU - Willen, Laure

AU - Tardivel, Aubry

AU - Smulski, Cristian R.

AU - Zheng, Timothy S.

AU - Gommerman, Jennifer

AU - Hess, Henry

AU - Gottenberg, Jacques Eric

AU - Mackay, Fabienne

AU - Donze, Olivier

AU - Schneider, Pascal

PY - 2016/9/16

Y1 - 2016/9/16

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AB - B cell activating factor of the TNF family (BAFF), also known as B lymphocyte stimulator, is a ligand required for the generation and maintenance of B lymphocytes. In this study, the ability of different monoclonal antibodies to recognize, inhibit, or activate mouse BAFF was investigated. One of them, a mouse IgG1 named Sandy-2, prevented the binding of BAFF to all of its receptors, BAFF receptor, transmembrane activator and calcium modulating ligand interactor, and B cell maturation antigen, at a stoichiometric ratio; blocked the activity of mouse BAFF on a variety of cell-based reporter assays; and antagonized the prosurvival action of BAFF on primary mouse B cells in vitro. A single administration of Sandy-2 in mice induced B cell depletion within 2 weeks, down to levels close to those observed in BAFF-deficient mice. This depletion could then be maintained with a chronic treatment. Sandy-2 and a previously described rat IgG1 antibody, 5A8, also formed a pair suitable for the sensitive detection of endogenous circulating BAFF by ELISA or using a homogenous assay. Interestingly, 5A8 and Sandy-5 displayed activities opposite to that of Sandy-2 by stimulating recombinant BAFF in vitro and endogenous BAFF in vivo. These tools will prove useful for the detection and functional manipulation of endogenous mouse BAFF and provide an alternative to the widely used BAFF receptor-Fc decoy receptor for the specific depletion of BAFF in mice.

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U2 - 10.1074/jbc.M116.725929

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