Antibiotic screening for IMP, VIM, OXA, KPC type Carbapenemases

Carbapenemases are a diverse group of β -lactamases that are active not only against the oxyiminocephalosporins and cephamycins but also against the carbapenems. Aztreonam is stable to the metallo-β-lactamases, but many IMP and VIM producers are resistant, owing to other mechanisms. Carbapenemases were formerly believed to derive only from classes A, B, and D. IMP type was screened from bacteria Pseudomonas and A cinetobacter species. VIM from P. aeruginosa, KPC from Klebsiella pneumoniae and OXA from Acinetobacter
species

Antibiotics Aminoglycosides (Gentamicin and Streptomycin), cefoxitin, aztreonam,trimethoprimsulfamethoxazole, fluoroquinolone, clavulanate, sulbactam, and tazobactam were screened for the above set of Carbapenemases. It was found that the type IMP, VIM and OXA are moderately susceptible to aztreonam and Streptomycin and showed that 39.6% produced IMP, 17.2% VIM and 13.8% OXA. KPC type showed resistantce to all the antibiotics applied.