Antibiotic resistance in Staphylococcus aureus isolated at an Australian hospital between 1946 and 1981

M. T. Gillespie, J. W. May, R. A. Skurray

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Abstract

A total of 517 strains of Staphylococcus aureus isolated at a hospital in Melbourne, Australia between 1946 and 1981 was examined for resistance to a range of antimicrobial agents and for the presence of plasmid DNA. The use of mixed-culture transfer and restriction endonuclease analysis showed that the determinants for resistance to penicillin and to the heavy metals were carried by several related plasmids of (15-23) x 106 mol. wt, and that tetracycline resistance was encoded on a plasmid of 2.8 x 106 mol.wt in strains isolated before 1970. These phenotypes were chromosomally encoded in the majority of strains isolated thereafter. Resistance to chloramphenicol throughout the study period was plasmid-mediated. Of five aminoglycoside-resistance phenotypes, one was plasmid-mediated and three were chromosomally encoded. The remaining phenotype, specifying low-level gentamicin resistance, was found to be located on the chromosome of early isolates, but in later strains was borne by an 18 x 106 mol.wt plasmid which also encoded resistance to quaternary ammonium compounds.

Original languageEnglish
Pages (from-to)137-147
Number of pages11
JournalJournal of Medical Microbiology
Volume19
Issue number2
DOIs
Publication statusPublished - 1 Jan 1985

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