Antiarthritic activity of Achyranthes aspera on formaldehyde-induced arthritis in rats

Velmurugan Chinnasamy, Vetriselvan Subramaniyan, Sarath Chandiran, Saminathan Kayarohanam, Dhanapal Сhidambaram Kanniyan, Venkata Sathya Saiappala Raju Velaga, Shafiulla Muhammad

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Abstract

To determine the ameliorative potential of aqueous extract of Achyranthesaspera(AEAA) against arthritis using swiss albino mice and Wistar rats, and its possible mechanism of action. METHODS: Swiss albino mice (25-30 g) and Wistar rats (150-180 g) under standard controlled conditions (24 ± 2°C, 50-70 humidity and 12 h light/dark cycle). The groups were divided into 6 groups (n = 6/group) and assigned as control, negative control, standard and, formaldehyde supplemented with two different test dose groups of A. aspera for 4 weeks. Arthritis induced by subplantar administration of 0.1 ml formaldehyde (2% v/v) into the left hind paw in all groups except normal control. Arthritis was assessed using serum Hb, ESR, paw volume, joint diameter, radiological and histopathological investigation. RESULTS: Oral administration of AEAA shown a significant (p < 0.01) dose-dependent protection against formaldehyde induced arthritis. At 21st day, A.asperashown an inhibition of paw volume in the different doses of 250 mg/kg and 500 mg/kg were found to be 30% and, 38.33% respectively. At 14th day the joint swelling was found to be 27.2% and 36.36 respectively. Diclofenac (10 mg/kg) had an effect of 36.61% inhibition of arthritis and joint swelling at 21st and 14th day. CONCLUSION: Thus, the present study revealed that the aqueous extract of A. aspera offered significant protection against arthritis and joint inflammation.

Original languageEnglish
Pages (from-to)2709-2714
Number of pages6
JournalOpen Access Macedonian Journal of Medical Sciences
Volume7
Issue number17
DOIs
Publication statusPublished - 15 Sept 2019
Externally publishedYes

Keywords

  • Achyranthesaspera
  • Anti-arthritis
  • Arthritis
  • Formaldehyde

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