ANTI-APOPTOTIC ACTIONS OF SAFINAMIDE IN 6-HYDROXYDOPAMINE-INDUCED CELL MODEL OF PARKINSON’S DISEASE

Mengge Shang, Yiing Jye Yap, Khuen Yen Ng, Soi Moi Chye, Anna Pick Kiong Ling, Rhun Yian Koh

Research output: Contribution to journalConference articleResearchpeer-review

Abstract

Parkinson’s disease (PD) is a progressive neurodegenerative disorder. Safinamide has been identified as a new therapeutic approach in the treatment of PD. In the present study, the effect of safinamide was studied on 6-hydroxydopamine (6-OHDA)-induced SHSY5Y cell model of PD. The 6-OHDA-challenged SH-SY5Y neuronal cells were treated with safinamide. Cell viability, reactive oxygen species (ROS) levels, and apoptosis were determined using dimethylthiazol-diphenyltetrazolium bromide (MTT) assay, dichloro-dihydro-fluorescein diacetate (DCFH-DA) assay and cell cycle analysis, respectively. Treatment with safinamide slightly increased the cell viability, lowered the ROS levels and reduced apoptosis. However, all the results obtained were not statistically significant. In conclusion, safinamide possesses slight neuroprotective properties, achieved through anti-oxidative stress and anti-apoptotic properties.

Original languageEnglish
Pages (from-to)58-63
Number of pages6
JournalMalaysian Journal of Biochemistry & Molecular Biology
Volume2022
Issue numberS1
Publication statusPublished - 2022
EventInternational Conference On Industry - Academia Initiatives In Biotechnology and Chemistry 2021 - Online
Duration: 20 Dec 202121 Dec 2021

Keywords

  • 6-Hydroxydopamine
  • Apoptosis
  • Neuron
  • Parkinson’s disease
  • Safinamide

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