Anterior pituitary cells from brattleboro (di/di), long-evans and sprague-dawley rats contain immunoreactive arginine vasopressin

S. J. Lolait, Alison J. Markwick, Margaret McNally, Josephine Abraham, A. Ian Smith, John W. Funder

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Abstract

A radioimmunoassay specific for arginine-vasopressin (AVP) was used to establish the presence of immunoreactive (ir)-AVP in extracts of anterior pituitary glands from Sprague-Dawley (SD) and Long-Evans (LE) rats (3.05 ± 1.0 and 1.66 ±0.9 ng/gland, respectively). Lower levels of ir-AVP (0.56 ±0.26 ng/gland) were detected in anterior pituitary gland extracts from rats with hereditary diabetes insipidus (Brattleboro; di/di). The anterior pituitary gland ir-AVP from each rat strain was further characterized by reverse-phase high-performance liquid chromatography (RP-HPLC). In each case the major peak of immunoreactivity co-migrated with synthetic AVP. By peroxidase-antiperoxidase immunocytochemistry, sparsely distributed cells containing ir-AVP were localized in anterior pituitary sections. Levels of ir-AVP in primary cultures of anterior pituitary cells (from SD rats) increased from 52 ± 5 pg/106 cells at 2 days in vitro to 152 ± 17 pg/106 cells at 3 days; during this period 56 ± 6 pg/ml ir-AVP was secreted into the culture medium. Fewer than 1% of the cells in these cultures were immunostainable for AVP. These data indicate that the anterior pituitary gland of the Brattleboro, Long-Evans and Sprague-Dawley rat contains ir-AVP, and that there is synthesis and secretion of this peptide in primary cultures of anterior pituitary cells in vitro.

Original languageEnglish
Pages (from-to)577-583
Number of pages7
JournalNeuroendocrinology
Volume43
Issue number5
DOIs
Publication statusPublished - 1 Jan 1986
Externally publishedYes

Keywords

  • Anterior pituitary gland
  • Arginine-vasopressin
  • Brattleboro rat
  • Cell culture
  • High-performance liquid chromatography
  • Immunocytochemistry
  • Radioimmunoassay

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