Annexin-1 peptide Anx-1 2-26 protects adult rat cardiac myocytes from cellular injury induced by simulated ischaemia

Rebecca H. Ritchie, Jennifer M. Gordon, Owen L. Woodman, Anh H. Cao, Gregory J. Dusting

Research output: Contribution to journalArticleResearchpeer-review

26 Citations (Scopus)

Abstract

The anti-inflammatory properties of annexin-1 peptides have been largely ascribed to their powerful antineutrophil actions in vivo. We have recently reported that the N-terminal fragment of annexin-1, Anx-1 2-26, preserves contractile function of cardiac muscle in vitro. The aim of the present study was to determine if Anx-1 2-26 elicits protective actions specifically on the cardiac myocyte (in the absence of neutrophils), using a model of metabolic inhibition to simulate ischaemia. 2 Metabolic inhibition of cardiac myocytes (4 h incubation at 37°C in HEPES-containing buffer supplemented with 2-deoxy-D-glucose, D,L-lactic acid and pH adjusted to 6.5) followed by 2.5 h recovery in normal medium markedly increased creatine kinase (CK) and lactate dehydrogenase (LDH) levels by 179 ± 39 and 26 ± 7IU L -1 (both n = 40, P < 0.001), respectively. However, cellular injury was significantly decreased when Anx-1 2-26 (0.3 mu;M) was present during metabolic inhibition, CK by 74 ± 10% and LDH by 71 ± 6% (both n = 31, P < 0.001), respectively. 3 Boc 2 (10 μM), a nonselective formyl peptide receptor antagonist, present during metabolic inhibition, abolished the cardioprotective effect of Anx-1 2-26. 4 Addition of chelerythrine (10 μM), 5-hydroxydecanoate (500 μM) or SB202190 (1 μM) during metabolic inhibition also abolished Anx-1 2-26- induced cardioprotection. 5 Cellular injury induced by metabolic inhibition was also largely prevented when myocytes were incubated with Anx-1 2-26 for 5 min with 10 min recovery prior to the insult, or when Anx-1 2-26 was present only during the recovery period following drug-free metabolic inhibition. 6 In conclusion, the annexin-1 peptide Anx-1 2-26 potently prevents cardiac myocyte injury induced by metabolic inhibition, an action that was dependent at least in part on the activation of the formyl peptide receptor family of G-protein-coupled receptors, protein kinase C, p38 mitogen-activated protein kinase and ATP-sensitive potassium channels.

Original languageEnglish
Pages (from-to)495-502
Number of pages8
JournalBritish Journal of Pharmacology
Volume145
Issue number4
DOIs
Publication statusPublished - 1 Jun 2005
Externally publishedYes

Keywords

  • Annexin-1
  • Cardioprotection
  • Formyl peptide receptor
  • Lipocortin-1
  • Protein kinase C

Cite this

Ritchie, Rebecca H. ; Gordon, Jennifer M. ; Woodman, Owen L. ; Cao, Anh H. ; Dusting, Gregory J. / Annexin-1 peptide Anx-1 2-26 protects adult rat cardiac myocytes from cellular injury induced by simulated ischaemia. In: British Journal of Pharmacology. 2005 ; Vol. 145, No. 4. pp. 495-502.
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abstract = "The anti-inflammatory properties of annexin-1 peptides have been largely ascribed to their powerful antineutrophil actions in vivo. We have recently reported that the N-terminal fragment of annexin-1, Anx-1 2-26, preserves contractile function of cardiac muscle in vitro. The aim of the present study was to determine if Anx-1 2-26 elicits protective actions specifically on the cardiac myocyte (in the absence of neutrophils), using a model of metabolic inhibition to simulate ischaemia. 2 Metabolic inhibition of cardiac myocytes (4 h incubation at 37°C in HEPES-containing buffer supplemented with 2-deoxy-D-glucose, D,L-lactic acid and pH adjusted to 6.5) followed by 2.5 h recovery in normal medium markedly increased creatine kinase (CK) and lactate dehydrogenase (LDH) levels by 179 ± 39 and 26 ± 7IU L -1 (both n = 40, P < 0.001), respectively. However, cellular injury was significantly decreased when Anx-1 2-26 (0.3 mu;M) was present during metabolic inhibition, CK by 74 ± 10{\%} and LDH by 71 ± 6{\%} (both n = 31, P < 0.001), respectively. 3 Boc 2 (10 μM), a nonselective formyl peptide receptor antagonist, present during metabolic inhibition, abolished the cardioprotective effect of Anx-1 2-26. 4 Addition of chelerythrine (10 μM), 5-hydroxydecanoate (500 μM) or SB202190 (1 μM) during metabolic inhibition also abolished Anx-1 2-26- induced cardioprotection. 5 Cellular injury induced by metabolic inhibition was also largely prevented when myocytes were incubated with Anx-1 2-26 for 5 min with 10 min recovery prior to the insult, or when Anx-1 2-26 was present only during the recovery period following drug-free metabolic inhibition. 6 In conclusion, the annexin-1 peptide Anx-1 2-26 potently prevents cardiac myocyte injury induced by metabolic inhibition, an action that was dependent at least in part on the activation of the formyl peptide receptor family of G-protein-coupled receptors, protein kinase C, p38 mitogen-activated protein kinase and ATP-sensitive potassium channels.",
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Annexin-1 peptide Anx-1 2-26 protects adult rat cardiac myocytes from cellular injury induced by simulated ischaemia. / Ritchie, Rebecca H.; Gordon, Jennifer M.; Woodman, Owen L.; Cao, Anh H.; Dusting, Gregory J.

In: British Journal of Pharmacology, Vol. 145, No. 4, 01.06.2005, p. 495-502.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Annexin-1 peptide Anx-1 2-26 protects adult rat cardiac myocytes from cellular injury induced by simulated ischaemia

AU - Ritchie, Rebecca H.

AU - Gordon, Jennifer M.

AU - Woodman, Owen L.

AU - Cao, Anh H.

AU - Dusting, Gregory J.

PY - 2005/6/1

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N2 - The anti-inflammatory properties of annexin-1 peptides have been largely ascribed to their powerful antineutrophil actions in vivo. We have recently reported that the N-terminal fragment of annexin-1, Anx-1 2-26, preserves contractile function of cardiac muscle in vitro. The aim of the present study was to determine if Anx-1 2-26 elicits protective actions specifically on the cardiac myocyte (in the absence of neutrophils), using a model of metabolic inhibition to simulate ischaemia. 2 Metabolic inhibition of cardiac myocytes (4 h incubation at 37°C in HEPES-containing buffer supplemented with 2-deoxy-D-glucose, D,L-lactic acid and pH adjusted to 6.5) followed by 2.5 h recovery in normal medium markedly increased creatine kinase (CK) and lactate dehydrogenase (LDH) levels by 179 ± 39 and 26 ± 7IU L -1 (both n = 40, P < 0.001), respectively. However, cellular injury was significantly decreased when Anx-1 2-26 (0.3 mu;M) was present during metabolic inhibition, CK by 74 ± 10% and LDH by 71 ± 6% (both n = 31, P < 0.001), respectively. 3 Boc 2 (10 μM), a nonselective formyl peptide receptor antagonist, present during metabolic inhibition, abolished the cardioprotective effect of Anx-1 2-26. 4 Addition of chelerythrine (10 μM), 5-hydroxydecanoate (500 μM) or SB202190 (1 μM) during metabolic inhibition also abolished Anx-1 2-26- induced cardioprotection. 5 Cellular injury induced by metabolic inhibition was also largely prevented when myocytes were incubated with Anx-1 2-26 for 5 min with 10 min recovery prior to the insult, or when Anx-1 2-26 was present only during the recovery period following drug-free metabolic inhibition. 6 In conclusion, the annexin-1 peptide Anx-1 2-26 potently prevents cardiac myocyte injury induced by metabolic inhibition, an action that was dependent at least in part on the activation of the formyl peptide receptor family of G-protein-coupled receptors, protein kinase C, p38 mitogen-activated protein kinase and ATP-sensitive potassium channels.

AB - The anti-inflammatory properties of annexin-1 peptides have been largely ascribed to their powerful antineutrophil actions in vivo. We have recently reported that the N-terminal fragment of annexin-1, Anx-1 2-26, preserves contractile function of cardiac muscle in vitro. The aim of the present study was to determine if Anx-1 2-26 elicits protective actions specifically on the cardiac myocyte (in the absence of neutrophils), using a model of metabolic inhibition to simulate ischaemia. 2 Metabolic inhibition of cardiac myocytes (4 h incubation at 37°C in HEPES-containing buffer supplemented with 2-deoxy-D-glucose, D,L-lactic acid and pH adjusted to 6.5) followed by 2.5 h recovery in normal medium markedly increased creatine kinase (CK) and lactate dehydrogenase (LDH) levels by 179 ± 39 and 26 ± 7IU L -1 (both n = 40, P < 0.001), respectively. However, cellular injury was significantly decreased when Anx-1 2-26 (0.3 mu;M) was present during metabolic inhibition, CK by 74 ± 10% and LDH by 71 ± 6% (both n = 31, P < 0.001), respectively. 3 Boc 2 (10 μM), a nonselective formyl peptide receptor antagonist, present during metabolic inhibition, abolished the cardioprotective effect of Anx-1 2-26. 4 Addition of chelerythrine (10 μM), 5-hydroxydecanoate (500 μM) or SB202190 (1 μM) during metabolic inhibition also abolished Anx-1 2-26- induced cardioprotection. 5 Cellular injury induced by metabolic inhibition was also largely prevented when myocytes were incubated with Anx-1 2-26 for 5 min with 10 min recovery prior to the insult, or when Anx-1 2-26 was present only during the recovery period following drug-free metabolic inhibition. 6 In conclusion, the annexin-1 peptide Anx-1 2-26 potently prevents cardiac myocyte injury induced by metabolic inhibition, an action that was dependent at least in part on the activation of the formyl peptide receptor family of G-protein-coupled receptors, protein kinase C, p38 mitogen-activated protein kinase and ATP-sensitive potassium channels.

KW - Annexin-1

KW - Cardioprotection

KW - Formyl peptide receptor

KW - Lipocortin-1

KW - Protein kinase C

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DO - 10.1038/sj.bjp.0706211

M3 - Article

VL - 145

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EP - 502

JO - British Journal of Pharmacology

JF - British Journal of Pharmacology

SN - 1476-5381

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ER -