Animals in synchrotrons: Overcoming challenges for high-resolution, live, small-animal imaging

Martin Donnelley; David Parsons; Kaye Morgan; Karen Siu

doi:10.1063/1.3478192

Animals in synchrotrons: Overcoming challenges for high-resolution, live, small-animal imaging

Martin Donnelley, David Parsons, Kaye Morgan, Karen Siu

Research output: Chapter in Book/Report/Conference proceeding › Conference Paper › Research › peer-review

19 Citations (Scopus)

Abstract

Physiological studies in small animals can be complicated, but the complexity is increased dramatically when performing live-animal synchrotron X-ray imaging studies. Our group has extensive experience in high-resolution live-animal imaging at the Japanese SPring-8 synchrotron, primarily examining airways in two-dimensions. These experiments normally image an area of 1.8mm??1.2mm at a pixel resolution of 0.45 I?m and are performed with live, intact, anaesthetized mice. There are unique challenges in this experimental setting. Importantly, experiments must be performed in an isolated imaging hutch not specifically designed for small-animal imaging. This requires equipment adapted to remotely monitor animals, maintain their anesthesia, and deliver test substances while collecting images. The horizontal synchrotron X-ray beam has a fixed location and orientation that limits experimental flexibility.

Original language	English
Title of host publication	AIP Conference Proceedings
Editors	Karen K W Siu
Place of Publication	Melville New York USA
Publisher	American Institute of Physics
Pages	30 - 34
Number of pages	5
Volume	1266
DOIs	https://doi.org/10.1063/1.3478192
Publication status	Published - 2010
Event	AIP International Conference on Medical Applications of Synchrotron Radiation - Melbourne, Australia, Melbourne Vic, Australia Duration: 15 Feb 2010 → 18 Feb 2010 Conference number: 6th

Publication series

Name	AIP Conference Proceedings
ISSN (Print)	0094-243X

Conference

Conference	AIP International Conference on Medical Applications of Synchrotron Radiation
Country/Territory	Australia
City	Melbourne Vic
Period	15/02/10 → 18/02/10
Other	6TH INTERNATIONAL CONFERENCE ON MEDICAL APPLICATIONS OF SYNCHROTRON RADIATION Conference date: 15–18 February 2010 Location: Melbourne (Australia) ISBN: 978-0-7354-0813-5 Editors: Volume number: 1266 Published: Jul 23, 2010

Access to Document

10.1063/1.3478192

Cite this

@inproceedings{323649782f2f42a7a825d95a9a510aae,

title = "Animals in synchrotrons: Overcoming challenges for high-resolution, live, small-animal imaging",

abstract = "Physiological studies in small animals can be complicated, but the complexity is increased dramatically when performing live-animal synchrotron X-ray imaging studies. Our group has extensive experience in high-resolution live-animal imaging at the Japanese SPring-8 synchrotron, primarily examining airways in two-dimensions. These experiments normally image an area of 1.8mm??1.2mm at a pixel resolution of 0.45 I?m and are performed with live, intact, anaesthetized mice. There are unique challenges in this experimental setting. Importantly, experiments must be performed in an isolated imaging hutch not specifically designed for small-animal imaging. This requires equipment adapted to remotely monitor animals, maintain their anesthesia, and deliver test substances while collecting images. The horizontal synchrotron X-ray beam has a fixed location and orientation that limits experimental flexibility.",

author = "Martin Donnelley and David Parsons and Kaye Morgan and Karen Siu",

year = "2010",

doi = "10.1063/1.3478192",

language = "English",

volume = "1266",

series = "AIP Conference Proceedings",

publisher = "American Institute of Physics",

pages = "30 -- 34",

editor = "Siu, {Karen K W}",

booktitle = "AIP Conference Proceedings",

address = "United States of America",

note = "AIP International Conference on Medical Applications of Synchrotron Radiation ; Conference date: 15-02-2010 Through 18-02-2010",

}

Donnelley, M, Parsons, D, Morgan, K & Siu, K 2010, Animals in synchrotrons: Overcoming challenges for high-resolution, live, small-animal imaging. in KKW Siu (ed.), AIP Conference Proceedings. vol. 1266, AIP Conference Proceedings, American Institute of Physics, Melville New York USA, pp. 30 - 34, AIP International Conference on Medical Applications of Synchrotron Radiation, Melbourne Vic, Australia, 15/02/10. https://doi.org/10.1063/1.3478192

Animals in synchrotrons: Overcoming challenges for high-resolution, live, small-animal imaging. / Donnelley, Martin; Parsons, David; Morgan, Kaye et al.
AIP Conference Proceedings. ed. / Karen K W Siu. Vol. 1266 Melville New York USA: American Institute of Physics, 2010. p. 30 - 34 (AIP Conference Proceedings).

Research output: Chapter in Book/Report/Conference proceeding › Conference Paper › Research › peer-review

TY - GEN

T1 - Animals in synchrotrons: Overcoming challenges for high-resolution, live, small-animal imaging

AU - Donnelley, Martin

AU - Parsons, David

AU - Morgan, Kaye

AU - Siu, Karen

N1 - Conference code: 6th

PY - 2010

Y1 - 2010

N2 - Physiological studies in small animals can be complicated, but the complexity is increased dramatically when performing live-animal synchrotron X-ray imaging studies. Our group has extensive experience in high-resolution live-animal imaging at the Japanese SPring-8 synchrotron, primarily examining airways in two-dimensions. These experiments normally image an area of 1.8mm??1.2mm at a pixel resolution of 0.45 I?m and are performed with live, intact, anaesthetized mice. There are unique challenges in this experimental setting. Importantly, experiments must be performed in an isolated imaging hutch not specifically designed for small-animal imaging. This requires equipment adapted to remotely monitor animals, maintain their anesthesia, and deliver test substances while collecting images. The horizontal synchrotron X-ray beam has a fixed location and orientation that limits experimental flexibility.

AB - Physiological studies in small animals can be complicated, but the complexity is increased dramatically when performing live-animal synchrotron X-ray imaging studies. Our group has extensive experience in high-resolution live-animal imaging at the Japanese SPring-8 synchrotron, primarily examining airways in two-dimensions. These experiments normally image an area of 1.8mm??1.2mm at a pixel resolution of 0.45 I?m and are performed with live, intact, anaesthetized mice. There are unique challenges in this experimental setting. Importantly, experiments must be performed in an isolated imaging hutch not specifically designed for small-animal imaging. This requires equipment adapted to remotely monitor animals, maintain their anesthesia, and deliver test substances while collecting images. The horizontal synchrotron X-ray beam has a fixed location and orientation that limits experimental flexibility.

UR - http://scitation.aip.org/getpdf/servlet/GetPDFServlet?filetype=pdf&id=APCPCS001266000001000030000001&idtype=cvips&prog=normal

U2 - 10.1063/1.3478192

DO - 10.1063/1.3478192

M3 - Conference Paper

VL - 1266

T3 - AIP Conference Proceedings

SP - 30

EP - 34

BT - AIP Conference Proceedings

A2 - Siu, Karen K W

PB - American Institute of Physics

CY - Melville New York USA

T2 - AIP International Conference on Medical Applications of Synchrotron Radiation

Y2 - 15 February 2010 through 18 February 2010

ER -

Animals in synchrotrons: Overcoming challenges for high-resolution, live, small-animal imaging

Abstract

Publication series

Conference

Access to Document

Other files and links

Cite this