Analyzing near wall sperm motion using TIRF microscopy

Reza Nosrati; David Sinton; Amine Driouchi; Christopher M. Yip

Analyzing near wall sperm motion using TIRF microscopy

Reza Nosrati, David Sinton, Amine Driouchi, Christopher M. Yip

Research output: Chapter in Book/Report/Conference proceeding › Conference Paper › Other › peer-review

Abstract

We measured swimming characteristics of bull sperm within 1000 nm film from a glass surface using total internal reflection fluorescence (TIRF) microscopy. Results indicate that in approximately 70% of the cases sperm follow counter-clockwise trajectories and the sperm head rotation is no longer observed.

Original language	English
Title of host publication	Proceedings of The Canadian Society for Mechanical Engineering International Congress 2014
Place of Publication	Ottawa Canada
Publisher	Canadian Society for Mechanical Engineering
Number of pages	2
Publication status	Published - 1 Jun 2014
Externally published	Yes
Event	Canadian Society for Mechanical Engineering International Congress 2014 - University of Toronto, Toronto, Canada Duration: 1 Jun 2014 → 4 Jun 2014

Conference

Conference	Canadian Society for Mechanical Engineering International Congress 2014
Abbreviated title	CSME2014
Country/Territory	Canada
City	Toronto
Period	1/06/14 → 4/06/14

Keywords

Sperm motility
Microswimmer
Surface Accumulation
Reproduction

Access to Document

248288516-oaFinal published version, 307 KB

Cite this

@inproceedings{56e6b394d43549a7a00dc9854b534c37,

title = "Analyzing near wall sperm motion using TIRF microscopy",

abstract = "We measured swimming characteristics of bull sperm within 1000 nm film from a glass surface using total internal reflection fluorescence (TIRF) microscopy. Results indicate that in approximately 70\% of the cases sperm follow counter-clockwise trajectories and the sperm head rotation is no longer observed.",

keywords = "Sperm motility, Microswimmer, Surface Accumulation, Reproduction",

author = "Reza Nosrati and David Sinton and Amine Driouchi and Yip, \{Christopher M.\}",

year = "2014",

month = jun,

day = "1",

language = "English",

booktitle = "Proceedings of The Canadian Society for Mechanical Engineering International Congress 2014",

publisher = "Canadian Society for Mechanical Engineering",

note = "Canadian Society for Mechanical Engineering International Congress 2014, CSME2014 ; Conference date: 01-06-2014 Through 04-06-2014",

}

Analyzing near wall sperm motion using TIRF microscopy. / Nosrati, Reza; Sinton, David; Driouchi, Amine et al.
Proceedings of The Canadian Society for Mechanical Engineering International Congress 2014. Ottawa Canada: Canadian Society for Mechanical Engineering, 2014. 104920.

Research output: Chapter in Book/Report/Conference proceeding › Conference Paper › Other › peer-review

TY - GEN

T1 - Analyzing near wall sperm motion using TIRF microscopy

AU - Nosrati, Reza

AU - Sinton, David

AU - Driouchi, Amine

AU - Yip, Christopher M.

PY - 2014/6/1

Y1 - 2014/6/1

N2 - We measured swimming characteristics of bull sperm within 1000 nm film from a glass surface using total internal reflection fluorescence (TIRF) microscopy. Results indicate that in approximately 70% of the cases sperm follow counter-clockwise trajectories and the sperm head rotation is no longer observed.

AB - We measured swimming characteristics of bull sperm within 1000 nm film from a glass surface using total internal reflection fluorescence (TIRF) microscopy. Results indicate that in approximately 70% of the cases sperm follow counter-clockwise trajectories and the sperm head rotation is no longer observed.

KW - Sperm motility

KW - Microswimmer

KW - Surface Accumulation

KW - Reproduction

M3 - Conference Paper

BT - Proceedings of The Canadian Society for Mechanical Engineering International Congress 2014

PB - Canadian Society for Mechanical Engineering

CY - Ottawa Canada

T2 - Canadian Society for Mechanical Engineering International Congress 2014

Y2 - 1 June 2014 through 4 June 2014

ER -