TY - JOUR
T1 - Analysis of Wolbachia protein synthesis in Drosophila in vivo
AU - Sasaki, T
AU - Braig, Henk
AU - O'Neill, Scott Leslie
PY - 1998
Y1 - 1998
N2 - Intracellular Wolbachia infections are extremely common in arthropods and exert profound control over the reproductive biology of the host. However, very little Is known about the underlying molecular mechanisms which mediate these interactions with the host. We examined protein synthesis by Wolbachia in a Drosophila host in vivo by selective metabolic labelling of prokaryotic proteins and subsequent analysis by 1D and 2D gel electrophoresis. Using this method we could identify the major proteins synthesized by Wolbachia in ovaries and testes of flies. Of these proteins the most abundant was of low molecular weight and showed size variation between Wolbachia strains which correlated with the reproductive phenotype they generated in flies. Using the gel systems we employed it was not possible to identify any proteins of Wolbachia origin in the mature sperm cells of infected flies.
AB - Intracellular Wolbachia infections are extremely common in arthropods and exert profound control over the reproductive biology of the host. However, very little Is known about the underlying molecular mechanisms which mediate these interactions with the host. We examined protein synthesis by Wolbachia in a Drosophila host in vivo by selective metabolic labelling of prokaryotic proteins and subsequent analysis by 1D and 2D gel electrophoresis. Using this method we could identify the major proteins synthesized by Wolbachia in ovaries and testes of flies. Of these proteins the most abundant was of low molecular weight and showed size variation between Wolbachia strains which correlated with the reproductive phenotype they generated in flies. Using the gel systems we employed it was not possible to identify any proteins of Wolbachia origin in the mature sperm cells of infected flies.
UR - http://onlinelibrary.wiley.com/doi/10.1046/j.1365-2583.1998.72057.x/pdf
U2 - 10.1046/j.1365-2583.1998.72057.x
DO - 10.1046/j.1365-2583.1998.72057.x
M3 - Article
VL - 7
SP - 101
EP - 105
JO - Insect Molecular Biology
JF - Insect Molecular Biology
SN - 0962-1075
IS - 2
ER -