Analysis of the incompatibility determinants of I-complex plasmids.

S. Nikoletti; P. Bird; J. Praszkier; J. Pittard

doi:10.1128/jb.170.3.1311-1318.1988

Analysis of the incompatibility determinants of I-complex plasmids.

S. Nikoletti, P. Bird, J. Praszkier, J. Pittard

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Abstract

The isolation and characterization of minireplicons corresponding to group B and I-complex plasmids are reported. The molecular structures of the small RNAs that may play a major role in the replication control and incompatibility reactions of the plasmids are compared. A mutant plasmid with changed copy number and incompatibility specificity is described. This mutant had a single-base-pair substitution in the DNA region that codes for the small RNA.

Original language	English
Pages (from-to)	1311-1318
Number of pages	8
Journal	Journal of Bacteriology
Volume	170
Issue number	3
DOIs	https://doi.org/10.1128/jb.170.3.1311-1318.1988
Publication status	Published - 1 Jan 1988
Externally published	Yes

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10.1128/jb.170.3.1311-1318.1988

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AB - The isolation and characterization of minireplicons corresponding to group B and I-complex plasmids are reported. The molecular structures of the small RNAs that may play a major role in the replication control and incompatibility reactions of the plasmids are compared. A mutant plasmid with changed copy number and incompatibility specificity is described. This mutant had a single-base-pair substitution in the DNA region that codes for the small RNA.

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Analysis of the incompatibility determinants of I-complex plasmids.

Abstract

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