Analysis of Secreted MIF from Cultured Murine Bone Marrow-Derived Immune Cells

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Abstract

Macrophage migration inhibitory factor (MIF) is expressed and released ubiquitously by numerous cell types and tissues. MIF is detected and constitutively expressed at the protein level both intra- and extracellularly. This chapter outlines methods for cultivating, purifying, detecting, and quantifying concentrations of MIF from murine primary derived macrophages and dendritic cell culture supernatants.
Original languageEnglish
Title of host publicationMacrophage Migration Inhibitory Factor
Subtitle of host publicationMethods and Protocols
EditorsJames Harris, Eric Morand
Place of PublicationNew York
PublisherHumana Press
Chapter4
Pages39-46
Number of pages8
Volume2080
ISBN (Electronic)9781493999361
ISBN (Print)9781493999354
DOIs
Publication statusPublished - 2020

Publication series

NameMethods in Molecular Biology: Springer Protocols
PublisherSpringer
Volume2080
Name
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • MIF
  • ELISA
  • Western blot
  • BMM
  • BMDC
  • Cell culture

Cite this

Lang, T. (2020). Analysis of Secreted MIF from Cultured Murine Bone Marrow-Derived Immune Cells. In J. Harris, & E. Morand (Eds.), Macrophage Migration Inhibitory Factor: Methods and Protocols (Vol. 2080, pp. 39-46). (Methods in Molecular Biology: Springer Protocols; Vol. 2080). Humana Press. https://doi.org/10.1007/978-1-4939-9936-1_4