@inbook{9f483c5a7cdb48c8b3b7bb172270767c,
title = "Analysis of Secreted MIF from Cultured Murine Bone Marrow-Derived Immune Cells",
abstract = "Macrophage migration inhibitory factor (MIF) is expressed and released ubiquitously by numerous cell types and tissues. MIF is detected and constitutively expressed at the protein level both intra- and extracellularly. This chapter outlines methods for cultivating, purifying, detecting, and quantifying concentrations of MIF from murine primary derived macrophages and dendritic cell culture supernatants.",
keywords = "MIF, ELISA, Western blot, BMM, BMDC, Cell culture",
author = "Tali Lang",
year = "2020",
doi = "10.1007/978-1-4939-9936-1_4",
language = "English",
isbn = "9781493999354",
volume = "2080",
series = "Methods in Molecular Biology: Springer Protocols",
publisher = "Humana Press",
pages = "39--46",
editor = "James Harris and Eric Morand",
booktitle = "Macrophage Migration Inhibitory Factor",
address = "United States of America",
}