Abstract
| Original language | English |
|---|---|
| Title of host publication | Macrophage Migration Inhibitory Factor |
| Subtitle of host publication | Methods and Protocols |
| Editors | James Harris, Eric Morand |
| Place of Publication | New York |
| Publisher | Humana Press |
| Chapter | 4 |
| Pages | 39-46 |
| Number of pages | 8 |
| Volume | 2080 |
| ISBN (Electronic) | 9781493999361 |
| ISBN (Print) | 9781493999354 |
| DOIs | |
| Publication status | Published - 2020 |
Publication series
| Name | Methods in Molecular Biology: Springer Protocols |
|---|---|
| Publisher | Springer |
| Volume | 2080 |
| Name | |
|---|---|
| ISSN (Print) | 1064-3745 |
| ISSN (Electronic) | 1940-6029 |
Keywords
- MIF
- ELISA
- Western blot
- BMM
- BMDC
- Cell culture
Cite this
}
Analysis of Secreted MIF from Cultured Murine Bone Marrow-Derived Immune Cells. / Lang, Tali.
Macrophage Migration Inhibitory Factor: Methods and Protocols. ed. / James Harris; Eric Morand. Vol. 2080 New York : Humana Press, 2020. p. 39-46 (Methods in Molecular Biology: Springer Protocols; Vol. 2080).Research output: Chapter in Book/Report/Conference proceeding › Chapter (Book) › Other › peer-review
TY - CHAP
T1 - Analysis of Secreted MIF from Cultured Murine Bone Marrow-Derived Immune Cells
AU - Lang, Tali
PY - 2020
Y1 - 2020
N2 - Macrophage migration inhibitory factor (MIF) is expressed and released ubiquitously by numerous cell types and tissues. MIF is detected and constitutively expressed at the protein level both intra- and extracellularly. This chapter outlines methods for cultivating, purifying, detecting, and quantifying concentrations of MIF from murine primary derived macrophages and dendritic cell culture supernatants.
AB - Macrophage migration inhibitory factor (MIF) is expressed and released ubiquitously by numerous cell types and tissues. MIF is detected and constitutively expressed at the protein level both intra- and extracellularly. This chapter outlines methods for cultivating, purifying, detecting, and quantifying concentrations of MIF from murine primary derived macrophages and dendritic cell culture supernatants.
KW - MIF
KW - ELISA
KW - Western blot
KW - BMM
KW - BMDC
KW - Cell culture
UR - https://www-springer-com.ezproxy.lib.monash.edu.au/gp/book/9781493999354
U2 - 10.1007/978-1-4939-9936-1_4
DO - 10.1007/978-1-4939-9936-1_4
M3 - Chapter (Book)
SN - 9781493999354
VL - 2080
T3 - Methods in Molecular Biology: Springer Protocols
SP - 39
EP - 46
BT - Macrophage Migration Inhibitory Factor
A2 - Harris, James
A2 - Morand, Eric
PB - Humana Press
CY - New York
ER -