Analysis of RNA-protein interactions by flow cytometry

Alexander S. Brodsky; Angus P.R. Johnston; Matt Trau; Pamela A. Silver

Analysis of RNA-protein interactions by flow cytometry

Alexander S. Brodsky, Angus P.R. Johnston, Matt Trau, Pamela A. Silver

Research output: Contribution to journal › Review Article › Research › peer-review

Abstract

Flow cytometry, in combination with advances in bead coding technologies, is maturing as a powerful high-throughput approach for analyzing molecular interactions. Applications of this technology include antibody assays and single nucleotide polymorphism mapping. This review describes the recent development of a microbead flow cytometric approach to analyze RNA-protein interactions and discusses emerging bead coding strategies that together will allow genome-wide identification of RNA-protein complexes. The microbead flow cytometric approach is flexible and provides new opportunities for functional genomic studies and small-molecule screening.

Original language	English
Pages (from-to)	235-240
Number of pages	6
Journal	Current Opinion in Molecular Therapeutics
Volume	5
Issue number	3
Publication status	Published - 1 Jun 2003
Externally published	Yes

Keywords

Flow cytometry
Functional genomics
Microbeads
Microspheres
RNA-protein interactions

Cite this

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title = "Analysis of RNA-protein interactions by flow cytometry",

abstract = "Flow cytometry, in combination with advances in bead coding technologies, is maturing as a powerful high-throughput approach for analyzing molecular interactions. Applications of this technology include antibody assays and single nucleotide polymorphism mapping. This review describes the recent development of a microbead flow cytometric approach to analyze RNA-protein interactions and discusses emerging bead coding strategies that together will allow genome-wide identification of RNA-protein complexes. The microbead flow cytometric approach is flexible and provides new opportunities for functional genomic studies and small-molecule screening.",

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AU - Brodsky, Alexander S.

AU - Johnston, Angus P.R.

AU - Trau, Matt

AU - Silver, Pamela A.

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Y1 - 2003/6/1

N2 - Flow cytometry, in combination with advances in bead coding technologies, is maturing as a powerful high-throughput approach for analyzing molecular interactions. Applications of this technology include antibody assays and single nucleotide polymorphism mapping. This review describes the recent development of a microbead flow cytometric approach to analyze RNA-protein interactions and discusses emerging bead coding strategies that together will allow genome-wide identification of RNA-protein complexes. The microbead flow cytometric approach is flexible and provides new opportunities for functional genomic studies and small-molecule screening.

AB - Flow cytometry, in combination with advances in bead coding technologies, is maturing as a powerful high-throughput approach for analyzing molecular interactions. Applications of this technology include antibody assays and single nucleotide polymorphism mapping. This review describes the recent development of a microbead flow cytometric approach to analyze RNA-protein interactions and discusses emerging bead coding strategies that together will allow genome-wide identification of RNA-protein complexes. The microbead flow cytometric approach is flexible and provides new opportunities for functional genomic studies and small-molecule screening.

KW - Flow cytometry

KW - Functional genomics

KW - Microbeads

KW - Microspheres

KW - RNA-protein interactions

UR - https://www.scopus.com/pages/publications/0141725447

M3 - Review Article

C2 - 12870432

AN - SCOPUS:0141725447

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EP - 240

JO - Current Opinion in Molecular Therapeutics

JF - Current Opinion in Molecular Therapeutics

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ER -

Analysis of RNA-protein interactions by flow cytometry

Abstract

Keywords

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Cite this