Analysis of RNA-protein interactions by flow cytometry

Alexander S. Brodsky, Angus P.R. Johnston, Matt Trau, Pamela A. Silver

Research output: Contribution to journalReview ArticleResearchpeer-review

Abstract

Flow cytometry, in combination with advances in bead coding technologies, is maturing as a powerful high-throughput approach for analyzing molecular interactions. Applications of this technology include antibody assays and single nucleotide polymorphism mapping. This review describes the recent development of a microbead flow cytometric approach to analyze RNA-protein interactions and discusses emerging bead coding strategies that together will allow genome-wide identification of RNA-protein complexes. The microbead flow cytometric approach is flexible and provides new opportunities for functional genomic studies and small-molecule screening.

Original languageEnglish
Pages (from-to)235-240
Number of pages6
JournalCurrent Opinion in Molecular Therapeutics
Volume5
Issue number3
Publication statusPublished - 1 Jun 2003
Externally publishedYes

Keywords

  • Flow cytometry
  • Functional genomics
  • Microbeads
  • Microspheres
  • RNA-protein interactions

Cite this

Brodsky, Alexander S. ; Johnston, Angus P.R. ; Trau, Matt ; Silver, Pamela A. / Analysis of RNA-protein interactions by flow cytometry. In: Current Opinion in Molecular Therapeutics. 2003 ; Vol. 5, No. 3. pp. 235-240.
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Analysis of RNA-protein interactions by flow cytometry. / Brodsky, Alexander S.; Johnston, Angus P.R.; Trau, Matt; Silver, Pamela A.

In: Current Opinion in Molecular Therapeutics, Vol. 5, No. 3, 01.06.2003, p. 235-240.

Research output: Contribution to journalReview ArticleResearchpeer-review

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